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TFPI2 Protein (His tag)

TFPI2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Catalog No. ABIN7198257
  • Target See all TFPI2 Proteins
    TFPI2 (Tissue Factor Pathway Inhibitor 2 (TFPI2))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    • 9
    • 4
    • 3
    • 2
    Human
    Source
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This TFPI2 protein is labelled with His tag.
    Purpose
    Recombinant Human TFPI2 Protein (His Tag)(Active)
    Sequence
    Met 1-Lys 213
    Characteristics
    A DNA sequence encoding the human TFPI2 (NP_006519.1) precursor (Met 1-Lys 213) was expressed with a C-terminal polyhistidine tag.
    Purity
    > 95 % as determined by reducing SDS-PAGE.
    Endotoxin Level
    < 1.0 EU per μg as determined by the LAL method.
    Biological Activity Comment
    Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114). The IC50 value is < 2 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15M NaCl , 0.05% Brij-35, pH 7.5.
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    Discover our top product TFPI2 Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Please refer to the printed manual for detailed information.
    Buffer
    Lyophilized from sterile PBS, pH 7.4
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target
    TFPI2 (Tissue Factor Pathway Inhibitor 2 (TFPI2))
    Alternative Name
    TFPI2 (TFPI2 Products)
    Synonyms
    MGC68843 Protein, TFPI2 Protein, MGC108301 Protein, si:ch211-262k23.2 Protein, tfpi2 Protein, PP5 Protein, REF1 Protein, TFPI-2 Protein, AV000670 Protein, PP5/TFPI-2 Protein, tissue factor pathway inhibitor 2 L homeolog Protein, tissue factor pathway inhibitor 2 Protein, tfpi2.L Protein, TFPI2 Protein, tfpi2 Protein, Tfpi2 Protein
    Background

    Background: Tissue factor pathway inhibitor-2 (TFPI2), a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor pathway inhibitor (TFPI). It is a 32 kDa matrix-associated glycoprotein consisting of a short amino-terminal region, three tandem Kunitz-type domains and a positively charged carboxy-terminal tail. TFPI2 inhibits plasmin-dependent activation of several metalloproteinases. TFPI2 is highly abundant in the full-term placenta and widely expressed in various adult human tissues, such as the liver, skeletal muscle, heart, kidney, and pancreas. The expression of TFPI2 in tumors is inversely related to an increasing degree of malignancy, which may suggest a role for TFPI2 in the maintenance of tumor stability and inhibition of the growth of neoplasms. TFPI2 inhibits the tissue factor/factor VIIa (TF/VIIa) complex and a wide variety of serine proteinases including plasmin, plasma kallikrein, factor XIa, trypsin, and chymotrypsin. TFPI2 is involved in regulating pericellular proteases implicated in a variety of physiologic and pathologic processes including cancer cell invasion, vascular inflammation, and atherosclerosis. TFPI2 has also been shown to induce apoptosis and inhibit angiogenesis, which may contribute significantly to tumor growth inhibition.

    Synonym: Tissue Factor Pathway Inhibitor 2, TFPI-2, Placental Protein 5, PP5, TFPI2

    Molecular Weight
    23.2 kDa
    NCBI Accession
    NP_006519
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