CD83 Protein (CD83) (AA 20-147) (His tag)
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- Target See all CD83 Proteins
- CD83
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-147
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Origin
- Cynomolgus
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD83 protein is labelled with His tag.
- Purpose
- Cynomolgus CD83 Protein
- Sequence
- Thr20-Glu147
- Characteristics
- Recombinant Cynomolgus CD83 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Glu147.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus CD83, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti- CD83 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD83
- Alternative Name
- CD83 (CD83 Products)
- Synonyms
- BL11 Protein, HB15 Protein, CD83 molecule Protein, CD83 antigen Protein, Cd83 Protein, CD83 Protein
- Background
- CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.
- Molecular Weight
- 15.43 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.
- UniProt
- A0A2K5US85
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