CD83 Protein (CD83) (AA 20-143) (Fc Tag)
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- Target See all CD83 Proteins
- CD83
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-143
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD83 protein is labelled with Fc Tag.
- Purpose
- Human CD83 Protein
- Sequence
- Thr20-Ala143
- Characteristics
- Recombinant Human CD83 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Thr20-Ala143.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD83, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD83 Antibody, hFc Tag with the EC50 21.3ng/ml determined by ELISA. See testing image for detail.
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CD83 (CD83) (AA 20-143) protein (His tag)
CD83 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD83 (CD83) (AA 20-147) protein (His tag)CD83 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD83 (CD83) (AA 22-134) protein (Fc Tag)CD83 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD83 (CD83) (AA 22-134) protein (His tag)CD83 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
CD83 (CD83) (AA 1-205) protein (GST tag)CD83 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD83 (CD83) protein (His tag)CD83 Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
CD83 (CD83) (AA 1-133) protein (His tag)CD83 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
CD83 (CD83) protein (Fc Tag)CD83 Origin: Rat Host: HEK-293 Cells Recombinant > 76 % as determined by SDS-PAGE
CD83 (CD83) (AA 20-144) protein (His tag)Crystallography grade CD83 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
CD83 (CD83) (AA 22-133) protein (His tag)Crystallography grade CD83 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD83
- Alternative Name
- CD83 (CD83 Products)
- Synonyms
- BL11 Protein, HB15 Protein, CD83 molecule Protein, CD83 antigen Protein, Cd83 Protein, CD83 Protein
- Background
- CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.
- Molecular Weight
- 40.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
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