M-CSF/CSF1 Protein (AA 33-190) (His-Avi Tag,Biotin)
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- Target See all M-CSF/CSF1 (CSF1) Proteins
- M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 33-190
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This M-CSF/CSF1 protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human M-CSF/CSF-1 Protein
- Sequence
- Glu33-Asn190
- Characteristics
- Recombinant Biotinylated Human M-CSF/CSF-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu33-Asn190.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human M-CSF R, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human M-CSF, His Tag with the EC50 of 61.3ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product CSF1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
- Alternative Name
- M-CSF (CSF1 Products)
- Synonyms
- CSF-1 Protein, MCSF Protein, C87615 Protein, Csfm Protein, op Protein, csf1-1 Protein, zgc:172186 Protein, CSF1 Protein, csf1-2 Protein, zgc:158436 Protein, colony stimulating factor 1 Protein, colony stimulating factor 1 (macrophage) Protein, colony stimulating factor 1a (macrophage) Protein, macrophage colony-stimulating factor 1 Protein, macrophage colony stimulating factor Protein, colony stimulating factor 1b (macrophage) Protein, CSF1 Protein, Csf1 Protein, csf1a Protein, LOC396599 Protein, LOC100860895 Protein, csf1b Protein
- Background
- CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.
- Molecular Weight
- 21.3 kDa. Due to glycosylation, the protein migrates to 27-37 kDa based on Tris-Bis PAGE result.
- Pathways
- RTK Signaling
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