Selectin E/CD62e Protein (AA 22-554) (His tag)

Catalog No. ABIN7274570

Product Details

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Protein Type: Recombinant

Protein Characteristics: AA 22-554

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This Selectin E/CD62e protein is labelled with His tag.

Purpose: Cynomolgus E-selectin/CD62E Protein

Sequence: Trp22-Pro554

Characteristics: Recombinant Cynomolgus E-selectin/CD62E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp22-Pro554.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus E-selectin, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 14.1ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: E-Selectin (SELE Products)

Synonyms: SELE Protein, CD62E Protein, ELAM Protein, ELAM1 Protein, ESEL Protein, LECAM2 Protein, E-selectin Protein, Elam Protein, selectin E Protein, selectin, endothelial cell Protein, SELE Protein, Sele Protein

Background: E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Molecular Weight: 59.47 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5U7H9

Pathways: Thromboxane A2 Receptor Signaling