Selectin E/CD62e Protein (AA 22-556) (His-Avi Tag)
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- Target See all Selectin E/CD62e (SELE) Proteins
- Selectin E/CD62e (SELE) (Selectin E (SELE))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-556
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Selectin E/CD62e protein is labelled with His-Avi Tag.
- Purpose
- Human E-Selectin/CD62E Protein
- Sequence
- Trp22-Pro556
- Characteristics
- Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp22-Pro556.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human E-Selectin at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail.
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Selectin E (SELE) (AA 22-556) protein (Fc Tag)
SELE Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Selectin E (SELE) (AA 22-554) protein (His tag)SELE Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Selectin E (SELE) protein (His-Avi Tag,Biotin)SELE Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Selectin E (SELE) (AA 22-557) protein (His tag)SELE Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Selectin E (SELE) (AA 1-610) protein (GST tag)SELE Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Selectin E (SELE) (AA 401-500) protein (GST tag)SELE Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Selectin E (SELE) (Active) protein (His tag)SELE Origin: Rat Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Selectin E (SELE) (Active) protein (Fc Tag)SELE Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Selectin E (SELE) (Active) protein (Fc Tag)SELE Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Selectin E (SELE) (Active) protein (His tag)SELE Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Selectin E/CD62e (SELE) (Selectin E (SELE))
- Alternative Name
- E-Selectin (SELE Products)
- Synonyms
- SELE Protein, CD62E Protein, ELAM Protein, ELAM1 Protein, ESEL Protein, LECAM2 Protein, E-selectin Protein, Elam Protein, selectin E Protein, selectin, endothelial cell Protein, SELE Protein, Sele Protein
- Background
- E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
- Molecular Weight
- 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
- UniProt
- P16581
- Pathways
- Thromboxane A2 Receptor Signaling
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