TNFRSF18 Protein (AA 26-161) (His-Avi Tag,Biotin)

Catalog No. ABIN7274788

Product Details

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Protein Type: Recombinant

Protein Characteristics: AA 26-161

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFRSF18 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human GITR/TNFRSF18 Protein

Sequence: Gln26-Glu161

Characteristics: Recombinant Biotinylated Human GITR/TNFRSF18 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln26-Glu161.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GITR Ligand Trimer, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GITR, His Tag with the EC50 of 0.14μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Alternative Name: GITR (TNFRSF18 Products)

Synonyms: GITR Protein, TNFRSF18 Protein, AITR Protein, Gitr Protein, CD357 Protein, GITR-D Protein, TNF receptor superfamily member 18 Protein, tumor necrosis factor receptor superfamily, member 18 Protein, TNFRSF18 Protein, Tnfrsf18 Protein

Background: GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Molecular Weight: 17.2 kDa. Due to glycosylation, the protein migrates to 26-28 kDa based on Tris-Bis PAGE result.

Pathways: Cancer Immune Checkpoints