IL-17A/F Protein (His-Avi Tag,Biotin)
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- Target See all IL-17A/F Proteins
- IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL-17A/F protein is labelled with His-Avi Tag,Biotin.
- Sequence
- Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Biotinylated Human IL-17A&F, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 0.19μg/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product IL-17A/F Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL-17A/F (Interleukin-17 A/F Heterodimer (IL-17A/F))
- Alternative Name
- IL-17A&F (IL-17A/F Products)
- Synonyms
- il17-5 Protein, si:ch211-63o20.2 Protein, interleukin 17a/f3 Protein, il17a/f3 Protein
- Background
- IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,IL24,ML-1,Interleukin-17F,Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
- Molecular Weight
- 15.1 kDa (IL-17A)&17.5 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa&27 kDa based on Tris-Bis PAGE result.
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