IL23R Protein (AA 24-355) (mFc Tag)

Catalog No. ABIN7275031

Product Details

Target: IL23R (Interleukin 23 Receptor (IL23R))

Protein Type: Recombinant

Protein Characteristics: AA 24-355

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL23R protein is labelled with mFc Tag.

Purpose: Human IL-23R Protein

Sequence: Gly24-Gly355

Characteristics: Recombinant Human IL-23R Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Gly24-Gly355.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-23 alpha&Mouse IL-12, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc with the EC50 of 0.36μg/ml determined by ELISA. The affinity constant of 11.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL23R (Interleukin 23 Receptor (IL23R))

Alternative Name: IL-23R (IL23R Products)

Synonyms: IL-23R Protein, IL23R Protein, il-23r Protein, interleukin 23 receptor Protein, IL23R Protein, il23r Protein, Il23r Protein

Background: Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R).This receptor associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade.

Molecular Weight: 63.7 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Tris-Bis PAGE result.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation