L1CAM Protein (AA 20-1120) (His tag)
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- Target See all L1CAM Proteins
- L1CAM (L1 Cell Adhesion Molecule (L1CAM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-1120
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This L1CAM protein is labelled with His tag.
- Purpose
- Human L1CAM Protein
- Sequence
- Ile20-Glu1120
- Characteristics
- Recombinant Human L1CAM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile20-Glu1120.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human L1CAM, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-L1CAM Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- L1CAM (L1 Cell Adhesion Molecule (L1CAM))
- Alternative Name
- L1CAM (L1CAM Products)
- Synonyms
- l1cam-a Protein, CAML1 Protein, CD171 Protein, HSAS Protein, HSAS1 Protein, MASA Protein, MIC5 Protein, N-CAM-L1 Protein, N-CAML1 Protein, NCAM-L1 Protein, S10 Protein, SPG1 Protein, L1 Protein, Hsas Protein, Hyd Protein, NCAML1 Protein, L1 cell adhesion molecule S homeolog Protein, L1 cell adhesion molecule Protein, l1cam.S Protein, L1CAM Protein, L1cam Protein
- Background
- L1 cell adhesion molecule (L1CAM) is one of the first neural adhesion molecules described with important functions in the development of the nervous system. Subsequent work discovered that L1CAM is expressed in many human cancers and is often associated with bad prognosis. This is most likely due to the motility and invasion promoting function of L1CAM. L1CAM is a valuable diagnostic/prognostic marker and an attractive target for the therapy of several human cancers.
- Molecular Weight
- 124.6 kDa. Due to glycosylation, the protein migrates to 150-180 kDa based on Tris-Bis PAGE result.
- Pathways
- Synaptic Membrane
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