MMP8 Protein (AA 21-467) (His tag)

Catalog No. ABIN7275265

Product Details

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Protein Type: Recombinant

Protein Characteristics: AA 21-467

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This MMP8 protein is labelled with His tag.

Purpose: Cynomolgus MMP-8 Protein

Sequence: Phe21-Ser467

Characteristics: Recombinant Cynomolgus MMP-8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe21-Ser467.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus MMP-8, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 38.2ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Alternative Name: MMP-8 (MMP8 Products)

Synonyms: MMP8 Protein, BB138268 Protein, CLG1 Protein, HNC Protein, MMP-8 Protein, PMNL-CL Protein, matrix metallopeptidase 8 Protein, neutrophil collagenase Protein, MMP8 Protein, Mmp8 Protein, LOC100339790 Protein

Background: Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases, including dilated cardiomyopathy (DCM), with the significance of surrogate markers of extracellular matrix (ECM) remodeling. MMP-8 was identified only in myocardiocytes, while MMP-9 and TIMP-2 were present in both myocardiocytes and stroma, but with different intensity. The increasing intensity of MMP-8 and TIMP-2 immunoreactions was significantly associated with low HCS.

Molecular Weight: 52.27 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005579523