MASP2 Protein (AA 287-686) (His tag)
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- Target See all MASP2 Proteins
- MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 287-686
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Origin
- Cynomolgus
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MASP2 protein is labelled with His tag.
- Purpose
- Cynomolgus MASP2 Protein
- Sequence
- Thr287-Phe686
- Characteristics
- Recombinant Cynomolgus MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Thr287-Phe686.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus MASP2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product MASP2 Protein
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Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 288-686) protein (His tag)
MASP2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag)MASP2 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag,Biotin)MASP2 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 287-685) protein (His tag)MASP2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 1-686) protein (GST tag)MASP2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 445-686) protein (His tag)Crystallography grade MASP2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 20-443) protein (His tag)Crystallography grade MASP2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 444-685) protein (His tag)Crystallography grade MASP2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 16-444) protein (His tag)Crystallography grade MASP2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Mannan-Binding Lectin serine Peptidase 2 (MASP2) (AA 16-686) protein (His tag)Crystallography grade MASP2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM Tris, 200 mM NaCl ( pH 9.0). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- MASP2 (Mannan-Binding Lectin serine Peptidase 2 (MASP2))
- Alternative Name
- MASP2 (MASP2 Products)
- Synonyms
- MAP19 Protein, MASP-2 Protein, MASP1P1 Protein, sMAP Protein, MAp19 Protein, mannan binding lectin serine peptidase 2 Protein, mannan-binding lectin serine peptidase 2 Protein, MASP2 Protein, Masp2 Protein
- Background
- The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19.
- Molecular Weight
- 46.8 kDa. Due to autocatalytic cleavage, the protein migrates to 21-24 kDa&28-30 kDa based on Tris-Bis PAGE result.
- UniProt
- A0A2K5UJY0
- Pathways
- Complement System
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