TNFRSF11A Protein (AA 30-212) (Fc Tag)

Catalog No. ABIN7275521

Product Details

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Protein Type: Recombinant

Protein Characteristics: AA 30-212

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFRSF11A protein is labelled with Fc Tag.

Purpose: Human TNFRSF11A/Rank Protein

Sequence: Ile30-Pro212

Characteristics: Recombinant Human TNFRSF11A/Rank Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile30-Pro212.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human RANKL, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Alternative Name: TNFRSF11A (TNFRSF11A Products)

Synonyms: RANK Protein, RGD1563614 Protein, TNFRSF11A Protein, Ly109 Protein, ODFR Protein, OFE Protein, Rank Protein, TRANCE-R Protein, mRANK Protein, CD265 Protein, FEO Protein, LOH18CR1 Protein, OPTB7 Protein, OSTS Protein, PDB2 Protein, TRANCER Protein, TNF receptor superfamily member 11A Protein, TNF receptor superfamily member 11a Protein, tumor necrosis factor receptor superfamily, member 11a, NFKB activator Protein, Tnfrsf11a Protein, TNFRSF11A Protein

Background: TNFRSF11A, also known as receptor activator of NF-κB (RANK), activates several signaling pathways, such as NF-κB, JNK, ERK, p38α, and Akt/PKB. RANK/TNFRSF11A is a novel and frequent target for de novo methylation in gliomas, which affects apoptotic activity and focus formation thereby contributing to the molecular pathogenesis of gliomas.

Molecular Weight: 46.85 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

Pathways: NF-kappaB Signaling