CD22 Protein (AA 20-687) (Fc Tag)

Catalog No. ABIN7275595

Product Details

Target: CD22 (CD22 Molecule (CD22))

Protein Type: Recombinant

Protein Characteristics: AA 20-687

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD22 protein is labelled with Fc Tag.

Purpose: Human Siglec-2/CD22 Protein

Sequence: Asp20-Arg687

Characteristics: Recombinant Human Siglec-2/CD22 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp20-Arg687.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Siglec-2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 46.6ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: Siglec2 (CD22 Products)

Synonyms: A530093D23 Protein, Lyb-8 Protein, Lyb8 Protein, SIGLEC-2 Protein, SIGLEC2 Protein, siglec-2 Protein, CD22 antigen Protein, CD22 molecule Protein, Cd22 Protein, CD22 Protein

Background: CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice.

Molecular Weight: 101.9 kDa. Due to glycosylation, the protein migrates to 120-130 kDa based on Tris-Bis PAGE result.