TGFA Protein (AA 40-89) (Fc Tag)

Catalog No. ABIN7275716

Product Details

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Protein Type: Recombinant

Protein Characteristics: AA 40-89

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TGFA protein is labelled with Fc Tag.

Purpose: Human TGF-alpha Protein

Sequence: Val40-Ala89

Characteristics: Recombinant Human TGF-alpha Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Val40-Ala89.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. The affinity constant of 0.31 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris,100 mM NaCl ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGF-alpha (TGFA Products)

Synonyms: TFGA Protein, LOC100009482 Protein, TGF alpha Protein, TGFA Protein, wa-1 Protein, wa1 Protein, RATTGFAA Protein, TGFAA Protein, NV15606 Protein, si:ch211-10e8.7 Protein, transforming growth factor alpha Protein, transforming growth factor alpha L homeolog Protein, transforming growth factor-alpha Protein, transforming growth factor, alpha Protein, TGFA Protein, TGF-A Protein, Tgfa Protein, tgfa.L Protein, LOC100121300 Protein, LOC100009150 Protein, tgfa Protein

Background: Transforming growth factor-alpha (TGFA) has been proposed as a candidate gene in the etiology of nonsyndromic cleft lip with or without cleft palate (NS-CL/P) and of nonsyndromic cleft palate only (NS-CPO). Biologic support for a role of TGFA arises from its presence at high levels in the epithelial tissue of the medial edge of the palatal shelves at the time of shelf fusion in mice.

Molecular Weight: 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway