TLR3 Protein (AA 23-703) (His tag)
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- Target See all TLR3 Proteins
- TLR3 (Toll-Like Receptor 3 (TLR3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-703
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TLR3 protein is labelled with His tag.
- Purpose
- Human TLR3 Protein
- Sequence
- Ser23-Glu703
- Characteristics
- Recombinant Human TLR3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser23-Glu703.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human TLR3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TLR3 Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
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Toll-Like Receptor 3 (TLR3) (AA 25-705) protein (His tag)
TLR3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Toll-Like Receptor 3 (TLR3) (AA 31-711) protein (His tag)TLR3 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Toll-Like Receptor 3 (TLR3) (AA 601-700) protein (GST tag)TLR3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Toll-Like Receptor 3 (TLR3) protein (Fc Tag)TLR3 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Toll-Like Receptor 3 (TLR3) (AA 23-703) protein (His tag,Biotin)TLR3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Toll-Like Receptor 3 (TLR3) (AA 726-904) protein (His tag)Crystallography grade TLR3 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Toll-Like Receptor 3 (TLR3) (AA 727-905) protein (His tag)Crystallography grade TLR3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Toll-Like Receptor 3 (TLR3) (AA 24-704) protein (His tag)Crystallography grade TLR3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Toll-Like Receptor 3 (TLR3) (AA 26-705) protein (His tag)Crystallography grade TLR3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Toll-Like Receptor 3 (TLR3) (AA 24-904) protein (rho-1D4 tag)Crystallography grade TLR3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- TLR3 (Toll-Like Receptor 3 (TLR3))
- Alternative Name
- TLR3 (TLR3 Products)
- Synonyms
- CD283 Protein, IIAE2 Protein, AI957183 Protein, zgc:123006 Protein, toll like receptor 3 Protein, toll-like receptor 3 Protein, TLR3 Protein, Tlr3 Protein, tlr3 Protein
- Background
- TLR3 is expressed in the central nervous system (CNS), where it is required to control HSV-1, which spreads from the epithelium to the CNS via cranial nerves. TLR3 is also expressed in epithelial and dendritic cells, which apparently use TLR3-independent pathways to prevent further dissemination of HSV-1 and to provide resistance to other pathogens in TLR3-deficient patients. Human TLR3 appears to be redundant in host defense to most microbes but is vital for natural immunity to HSV-1 in the CNS, which suggests that neurotropic viruses have contributed to the evolutionary maintenance of TLR3.
- Molecular Weight
- 78.7 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result.
- UniProt
- Q6PCD4
- Pathways
- TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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