ULBP2 Protein (AA 26-217) (His-Avi Tag)
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- Target See all ULBP2 Proteins
- ULBP2 (UL16 Binding Protein 2 (ULBP2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-217
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ULBP2 protein is labelled with His-Avi Tag.
- Purpose
- Human ULBP-2 Protein
- Sequence
- Gly26-Ser217
- Characteristics
- Recombinant Human ULBP-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly26-Ser217.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human ULBP-2, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.26μg/ml determined by ELISA. See testing image for detail.
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UL16 Binding Protein 2 (ULBP2) protein (His-Avi Tag,Biotin)
ULBP2 Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
UL16 Binding Protein 2 (ULBP2) (AA 25-224) protein (His tag)ULBP2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
UL16 Binding Protein 2 (ULBP2) (AA 1-246) protein (GST tag)ULBP2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
UL16 Binding Protein 2 (ULBP2) (AA 26-216) protein (His tag)Crystallography grade ULBP2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- ULBP2 (UL16 Binding Protein 2 (ULBP2))
- Alternative Name
- ULBP-2 (ULBP2 Products)
- Synonyms
- N2DL2 Protein, RAET1H Protein, UL16 binding protein 2 Protein, NKG2D ligand 2-like Protein, ULBP2 Protein, LOC100355436 Protein
- Background
- ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway, mediating natural killer cell cytotoxicity.
- Molecular Weight
- 24.6 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9BZM5
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