Claudin 18.2 (AA 1-261) (Active) protein-VLP

Catalog No. ABIN7448160

Product Details

Target: Claudin 18.2

Protein Type: VLP

Biological Activity: Active

Protein Characteristics: AA 1-261

Origin: Human

Source: HEK-293 Cells

Application: ELISA, Functional Studies (Func), Immunogen (Imm), Surface Plasmon Resonance (SPR)

Purpose: Human Claudin 18.2 Protein-VLP

Sequence: Met1-Val261

Characteristics: Full length Human Claudin 18.2 Protein-VLP is expressed from HEK293.It contains Met1-Val261.

Purity: > 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Claudin 18.2 VLP at 5μg/ml (100μl/Well). Dose response curve for Anti-Claudin 18.2 Antibody, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA (QC Test). The affinity constant of 2.20 nM as determined in SPR assay (Biacore T200).

Application Details

Application Notes:

• Antibody Discovery: Immunization, Screening, Functional Characterization
• Affinity determination: ELISA, SPR
• In vivo pharmacokinetic analysis
• CMC method development
• CAR-T Positive Rate Detection
• Blood sample determination: ELISA

Comment:

Virus-like particles (VLPs) are formed from the outer capsid protein of a virus and are tiny nanoparticles formed by the automatic assembly of one or more capsid proteins. VLPs do not contain viral infectious genomes, so they are relatively safe during production operations. The SAMS™ protein engineering platform has been used to express a series of biotinylated, non-biotinylated, and fluorescently-labeled VLP-displayed antigens. They are suitable for SPR, ELISA, CAR-T positive rate detection, and other experimental scenarios.

Virus-Like Particles (VLPs) are highly immunogenic, meaning that they can elicit a strong immune response in the host. VLPs are recognized by the immune system and are taken up by antigen-presenting cells (APCs) such as dendritic cells. Once taken up by APCs, VLPs are processed and presented to T cells, which can trigger the activation of B cells to produce antibodies against the displayed antigen. Because VLPs resemble the structure and composition of native viruses, they are highly effective at inducing both humoral and cellular immune responses.

Generally, VLPs range in size from approximately 20 to 200 nanometers (nm). Compared to a cell-based immunization approach, their smaller size can optimize the immune response to target the specific antigen displayed on the surface of the engineered VLPs.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in PBS ( pH 7.4). Notice: If you need it for immunization, Do Not use any adjuvant.

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Claudin 18.2

Background: Claudin18(CLDN18) belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells.CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants,CLDN18.1 and CLDN18.2. Isoform 2 (Claudin 18.2) is abundant in gastric tumors.

Molecular Weight: 29 kDa.