CLEC4C Protein (AA 46-213) (His tag)
CLEC4C
Origin: Human
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE
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- Target See all CLEC4C products
- CLEC4C (C-Type Lectin Domain Family 4, Member C (CLEC4C))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 46-213
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CLEC4C protein is labelled with His tag.
- Purpose
- Human BDCA-2 Protein
- Sequence
- Phe46-Ile213
- Characteristics
- Recombinant Human BDCA-2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Phe46-Ile213.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human BDCA-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). The affinity constant of 2.97 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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C-Type Lectin Domain Family 4, Member C (CLEC4C) (AA 45-213) protein (His-SUMO Tag)
CLEC4C Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as 0.22μm filtered solution in 20 mM Tris, 300 mM NaCl, 200 mM L-arginine ( pH 8.5).
- Storage
- -80 °C
- Storage Comment
- Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CLEC4C (C-Type Lectin Domain Family 4, Member C (CLEC4C))
- Alternative Name
- BDCA-2 (CLEC4C Products)
- Synonyms
- BDCA2 Protein, CD303 Protein, CLECSF11 Protein, CLECSF7 Protein, DLEC Protein, HECL Protein, PRO34150 Protein, Clec4c Protein, C-type lectin domain family 4 member C Protein, CLEC4C Protein, LOC100733227 Protein
- Background
- BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.
- Molecular Weight
- 20.93 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
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