Retinoic Acid Early Transcript 1E (RAET1E) (AA 31-225) protein (Fc Tag)

Catalog No. ABIN7454206

Product Details

Target: Retinoic Acid Early Transcript 1E (RAET1E)

Protein Type: Recombinant

Protein Characteristics: AA 31-225

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: Fc Tag

Purpose: Human ULBP-4 Protein

Sequence: His31-Asp225

Characteristics: Recombinant Human ULBP-4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains His31-Asp225.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.17 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Retinoic Acid Early Transcript 1E (RAET1E)

Alternative Name: ULBP-4 (RAET1E Products)

Synonyms: LETAL Protein, N2DL-4 Protein, NKG2DL4 Protein, RAET1E2 Protein, RL-4 Protein, ULBP4 Protein, bA350J20.7 Protein, retinoic acid early transcript 1E Protein, RAET1E Protein, Raet1e Protein

Background: UL16-binding proteins (ULBPs) are markers of cellular stress which are upregulated on the surface of virus-infected and tumor cells. ULBPs are expressed by a variety of leukemias, carcinomas, melanomas, and tumor cell lines.ULBP4 is associated with higher levels of CD8+/NKG2D+ lymphocytes infiltrating the carcinoma and is a prognosticator of improved survival.

Molecular Weight: 49.12 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.