HLAG Protein (Monomer) (Biotin,no Peptide)

Catalog No. ABIN7504418

Product Details

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Protein Type: Recombinant

Protein Characteristics: Monomer

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This HLAG protein is labelled with Biotin,no Peptide.

Purpose: Biotinylated Human HLA-G & B2M Monomer Protein

Sequence: Gly25-Thr305 (HLA-G) & Ile21-Met119 (B2M)

Specificity: Uni-Prot: P17693-1 (HLA-G) , P61769 (B2M)

Characteristics: Recombinant Biotinylated Human HLA-G & B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-G) and Ile21-Met119(B2M).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Application Notes: Can be used for Assess MHC/TCR Binding Affinity, generate custom MHC peptide monomer or tetramer, High Throughput Peptide Screening

Comment:

The HLA-G&B2M Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-G. The MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Alternative Name: HLA-G (HLAG Products)

Synonyms: MHC-G Protein, B-F Protein, B-F-S04 Protein, B-F-S05 Protein, B-F-S06 Protein, B-F-S07 Protein, B-FI Protein, B-FIV Protein, BF2 Protein, BFa2 Protein, BFw-03 Protein, BFw-05 Protein, BFz-01 Protein, major histocompatibility complex, class I, G Protein, MHC BF1 class I Protein, HLA-G Protein, BF1 Protein

Molecular Weight: 48.40 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints