ITGAV/ITGB1 Protein (AA 31-992, Heterodimer) (His-Avi Tag)

Catalog No. ABIN7504451

Product Details

Target: ITGAV/ITGB1 (Integrin alpha V + beta 1 (ITGAV/ITGB1))

Protein Type: Recombinant

Protein Characteristics: AA 31-992, Heterodimer

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ITGAV/ITGB1 protein is labelled with His-Avi Tag.

Purpose: Human Integrin alpha V beta 1 (ITGAV & ITGB1) Heterodimer Protein

Sequence: Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail

Specificity: Uni-Prot: P06756-1 (ITGAV), P05556-1 (ITGB1)

Characteristics: Recombinant Human Integrin alpha V beta 1 (ITGAV & ITGB1) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992 (ITGAV) acidic tail & Gln21-Asp728 (ITGB1) basic tail.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ITGAV/ITGB1 (Integrin alpha V + beta 1 (ITGAV/ITGB1))

Alternative Name: Integrin alpha V beta 1 (ITGAV/ITGB1 Products)

Background: Integrins are transmembrane heterodimeric receptors that contribute to diverse biological functions and play a conserved role in mediating cell adhesion to fixed extracellular ligands and in the maintenance of tissue integrity. αVβ1 is the major integrin on several different primary fibroblasts responsible for binding to TGFb1 LAP and for mediating activation of latent TGFb by these cells.

Molecular Weight: 114 kDa (ITGAV) and 83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 130-160 kDa (ITGAV) and 100-120 kDa (ITGB1) based on Tris-Bis PAGE result.