RNF43 Protein (AA 24-197) (His tag)
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- Target See all RNF43 Proteins
- RNF43 (Ring Finger Protein 43 (RNF43))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-197
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This RNF43 protein is labelled with His tag.
- Purpose
- Human RNF43 Protein
- Sequence
- Gly24-Tyr197
- Characteristics
- Recombinant Human RNF43 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly24-Tyr197.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Top Product
- Discover our top product RNF43 Protein
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Ring Finger Protein 43 (RNF43) (AA 44-197) (Active) protein (Fc Tag)
RNF43 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Ring Finger Protein 43 (RNF43) (AA 24-197) protein (His tag)Crystallography grade RNF43 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Ring Finger Protein 43 (RNF43) (AA 24-197) protein (His tag)Crystallography grade RNF43 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Ring Finger Protein 43 (RNF43) (AA 219-783) protein (His tag)Crystallography grade RNF43 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Ring Finger Protein 43 (RNF43) (AA 219-784) protein (His tag)Crystallography grade RNF43 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Ring Finger Protein 43 (RNF43) (AA 24-783) protein (rho-1D4 tag)Crystallography grade RNF43 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Ring Finger Protein 43 (RNF43) (AA 24-784) protein (rho-1D4 tag)Crystallography grade RNF43 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- RNF43 (Ring Finger Protein 43 (RNF43))
- Alternative Name
- RNF43 (RNF43 Products)
- Synonyms
- RNF124 Protein, URCC Protein, 4732452J19Rik Protein, RGD1305204 Protein, ring finger protein 43 Protein, RNF43 Protein, Rnf43 Protein
- Background
- RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
- Molecular Weight
- 20.14 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result.
- Pathways
- WNT Signaling
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