BIM Protein (AA 1-120) (His tag)

Catalog No. ABIN7504929

Product Details

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Protein Type: Recombinant

Protein Characteristics: AA 1-120

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This BIM protein is labelled with His tag.

Sequence: Met1-Arg120

Characteristics: Recombinant Human Bcl-2-like Protein 11 is produced by our E.coli expression system and the target gene encoding Met1-Arg120 is expressed with a 6His tag at the N-terminus.

Purity: >90 % as determined by reducing SDS-PAGE.

Endotoxin Level: <1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4., 5 % trehalose, 5 % mannitol, 0.01 % tween-80.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Alternative Name: BIM (BCL2L11 Products)

Synonyms: bim Protein, BAM Protein, BIM Protein, BOD Protein, 1500006F24Rik Protein, Bim Protein, Bod Protein, BimL Protein, BIMEL Protein, BCL2-like 11 Protein, BCL2 like 11 Protein, BCL2-like 11 (apoptosis facilitator) Protein, bcl2l11 Protein, BCL2L11 Protein, Bcl2l11 Protein

Background:

Background: BIML is one of several splice variants of BIM, a proapoptotic protein belonging to the BH-3 domain-only subgroup of Bcl-2 family members. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BIML is thought to promote apoptosis by binding and inhibiting the activity of anti-apoptotic Bcl-2 family members, thereby inducing the release of cytochrome c from mitochondria. BIML is normally sequestered in an inactive conformation from anti-apoptotic Bcl-2 family members through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release BIML from microtubules to neutralize anti-apoptotic Bcl-2 family members, allowing for the initiation of apoptosis.

Synonym: Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BCL2L11, BIM, BIML

Molecular Weight: 15 kDa

Pathways: PI3K-Akt Signaling, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity