AGT Protein (His tag)

Catalog No. ABIN7519680

Product Details

Target: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This AGT protein is labelled with His tag.

Purpose: Recombinant Mouse Angiotensinogen/AGT Protein

Sequence: GDRVYIHPFH LLYHNKSTCA QLENPSVETL PESTFEPVPI QAKTSPVNEK TLHDQLVLAA EKLEDEDRKR AAQVAMIANF VGFRMYKMLN EAGSGASGAI LSPPALFGTL VSFYLGSLDP TASQLQTLLD VPVKEGDCTS RLDGHKVLAA LRAIQGLLVT QGGSSSQTPL LQSIVVGLFT APGFRLKHSF VQSLALFTPA LFPRSLDLST DPVLATEKIN RFIKAVTGWK MNLPLEGVST DSTLLFNTYV HFQGTMRGFS QLPGVHEFWV DNSISVSVPM ISGTGNFQHW SDTQNNFSVT CVPLGERATL LLIQPHCTSD LDRVEALIFR NDLLTWIENP PPRAIRLTLP QLEIRGSYNL QDLLAEDKLP TLLGAEANLN NIGDTNPRVG EVLNSILLEL KAGEEEQPTT SVQQPGSPEA LDVTLSSPFL FAIYEQDSGT LHFLGRVNNP QSVV

Specificity: Gly29-Val482

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: <0.1EU/μg

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: AGT (Angiotensinogen (serpin Peptidase Inhibitor, Clade A, Member 8) (AGT))

Alternative Name: Angiotensinogen/AGT (AGT Products)

Synonyms: ANHU Protein, SERPINA8 Protein, AI265500 Protein, AngI Protein, AngII Protein, Aogen Protein, Serpina8 Protein, ANRT Protein, Ang Protein, PAT Protein, wu:fb62f06 Protein, wu:fj87b02 Protein, zgc:111892 Protein, AGT Protein, angt Protein, ANGT Protein, angiotensinogen Protein, angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Protein, AGT Protein, Agt Protein, agt Protein

Background:

Description: Angiotensinogen, also known as AGT and SerpinA8, is a member of the serpin family. It is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. Angiotensinogen can be schematically considered to consist of a combination of an angiotensin I (Ang I) function, located at the N-terminal end, and the presence of a serpin (serine protease inhibitor) structure at the opposite end. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system.

Name: AngI, AngII, Aogen, Serpina8,AGT

Gene ID: 11606

UniProt: Q3UTR7

Pathways: JAK-STAT Signaling, ACE Inhibitor Pathway, EGFR Signaling Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Feeding Behaviour, Monocarboxylic Acid Catabolic Process, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Regulation of long-term Neuronal Synaptic Plasticity