BMPR2 Protein (Fc Tag)

Catalog No. ABIN7535505

Product Details

Target: BMPR2 (Bone Morphogenetic Protein Receptor, Type II (serine/threonine Kinase) (BMPR2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This BMPR2 protein is labelled with Fc Tag.

Purpose: Active Recombinant Human BMPR-2 Protein

Sequence: SQNQERLCAF KDPYQQDLGI GESRISHENG TILCSKGSTC YGLWEKSKGD INLVKQGCWS HIGDPQECHY EECVVTTTPP SIQNGTYRFC CCSTDLCNVN FTENFPPPDT TPLSPPHSFN RDETI

Specificity: Ser27-Ile151

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human BMP2 at 1 μg/mL (100 μL/well) can bind Human BMPR2 with a linear range of 19.5 ng/ml-1.46 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: BMPR2 (Bone Morphogenetic Protein Receptor, Type II (serine/threonine Kinase) (BMPR2))

Alternative Name: BMPR-2 (BMPR2 Products)

Synonyms: BMPR-II Protein, BMPR3 Protein, BMR2 Protein, BRK-3 Protein, PPH1 Protein, T-ALK Protein, 2610024H22Rik Protein, AL117858 Protein, AW546137 Protein, BB189135 Protein, BMP-2 Protein, BMPR-2 Protein, BMPRII Protein, Gm20272 Protein, Bmpr-II Protein, bmprii Protein, bone morphogenetic protein receptor type 2 Protein, bone morphogenetic protein receptor, type II b (serine/threonine kinase) Protein, bone morphogenetic protein receptor, type II (serine/threonine kinase) Protein, BMPR2 Protein, bmpr2b Protein, Bmpr2 Protein

Background:

Description: The bone morphogenetic protein type II receptor (BMPR-II, or BMPR2), a receptor for the transforming growth factor (TGF)-beta/bone morphogenetic protein (BMP) superfamily. Reduced expression or function of BMPR2 signaling leads to exaggerated TGF-beta signaling and altered cellular responses to TGF-beta. In endothelial cells, BMPR2 mutation increases the susceptibility of cells to apoptosis. BMPR2 transduces BMP signals by forming heteromeric complexes with and phosphorylating BMP type I receptors. The intracellular domain of BMPR2 is both necessary and sufficient for receptor complex interaction. It had been identified that BMPR2 plays a key role in cell growth. Its mutations lead to hereditary pulmonary hypertension, and knockout of Bmpr-II results in early embryonic lethality. The C-terminal tail of BMPR2 provides binding sites for a number of regulatory proteins that may initiate Smad-independent signalling. BMPR2 mutations were predicted to alter the BMP and TGF-b1/SMAD signalling pathways, resulting in proliferation rather than apoptosis of vascular cells, and greatly increase the risk of developing severe pulmonary arterial hypertension. BMPR2 gene result in familial Primary pulmonary hypertension (PPH) transmitted as an autosomal dominant trait, albeit with low penetrance. Heterozygous germline mutations of BMPR2 gene have been identified in patients with familial and sporadic PPH, indicating that BMPR2 may contribute to the maintenance of normal pulmonary vascular structure and function. Tctex-1, a light chain of the motor complex dynein, interacts with the cytoplasmic domain of BMPR2 and demonstrate that Tctex-1 is phosphorylated by BMPR-II, a function disrupted by PPH disease causing mutations within exon 12. BMPR2 and Tctex-1 co-localize to endothelium and smooth muscle within the media of pulmonary arterioles, key sites of vascular remodelling in PPH.

Name: BMPR2, BMPR-II, BMPR3, BMR2, BRK-3, POVD1, PPH1, T-ALK, bone morphogenetic protein receptor type-2,BMPR-II,BMPR3,BMR2,BRK-3,POVD1,PPH1,T-ALK

Gene ID: 659

UniProt: Q13873-1

Pathways: Growth Factor Binding