FCGR2A Protein (His167Arg) (His tag)

Catalog No. ABIN7533897

Product Details

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: His167Arg

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FCGR2A protein is labelled with His tag.

Purpose: Active Recombinant Human Fc-gamma RIIa/CD32a(H167R) Protein

Sequence: AAPPKAVLKL EPPWINVLQE DSVTLTCQGA RSPESDSIQW FHNGNLIPTH TQPSYRFKAN NNDSGEYTCQ TGQTSLSDPV HLTVLSEWLV LQTPHLEFQE GETIMLRCHS WKDKPLVKVT FFQNGKSQKF SHLDPTFSIP QANHSHSGDY HCTGNIGYTL FSSKPVTITV QVPSMGSSSP MGI

Specificity: Ala36-Ile218(H167R)

Purity: > 97 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD32a at 1 μg/mL (100 μL/well) can bind biotinylated human IgG1 with a linear range of 30-250 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Alternative Name: Fc-gamma RIIa/CD32a (FCGR2A Products)

Synonyms: CD32 Protein, CD32A Protein, CDw32 Protein, FCG2 Protein, FCGR2 Protein, FCGR2A1 Protein, FcGR Protein, IGFR2 Protein, CD32a Protein, Fc fragment of IgG receptor IIa Protein, FCGR2A Protein

Background:

Description: The member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

Name: FCGR2A,CD32,CD32A,CDw32,FCG2,FCGR2,FCGR2A1,FcGR,IGFR2

Gene ID: 2212

UniProt: P12318