Galectin 9 Protein (His tag)

Catalog No. ABIN7534733

Product Details

Target: Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Galectin 9 protein is labelled with His tag.

Purpose: Active Recombinant Human Galectin-9/LGALS9 Protein

Sequence: AFSGSQAPYL SPAVPFSGTI QGGLQDGLQI TVNGTVLSSS GTRFAVNFQT GFSGNDIAFH FNPRFEDGGY VVCNTRQNGS WGPEERKTHM PFQKGMPFDL CFLVQSSDFK VMVNGILFVQ YFHRVPFHRV DTISVNGSVQ LSYISFQPPG VWPANPAPIT QTVIHTVQSA PGQMFSTPAI PPMMYPHPAY PMPFITTILG GLYPSKSILL SGTVLPSAQR FHINLCSGNH IAFHLNPRFD ENAVVRNTQI DNSWGSEERS LPRKMPFVRG QSFSVWILCE AHCLKVAVDG QHLFEYYHRL RNLPTINRLE VGGDIQLTHV QT

Specificity: Ala2-Thr323

Purity: > 92 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin-9 at 2 μg/mL can bind Recombinant Human TIM-3 with a linear range of 5-15 μg/mL.|2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8x104 cells/well are added to LGALS9 coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/ml PHA, approximately 70-80% cells will adhere specifically after 60 minutes at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))

Alternative Name: Galectin-9/LGALS9 (LGALS9 Products)

Synonyms: huat Protein, lgals9a Protein, ecalectin Protein, galectin-9 Protein, Lgals9 Protein, wu:fd20c09 Protein, zgc:111833 Protein, LGALS9 Protein, AA407335 Protein, AI194909 Protein, AI265545 Protein, LGALS35 Protein, Lgals5 Protein, gal-9 Protein, HUAT Protein, LGALS9A Protein, UAT Protein, UATP.I Protein, galectin 9 L homeolog Protein, galectin 9 Protein, lectin, galactoside-binding, soluble, 9 (galectin 9)-like 3 Protein, lectin, galactose binding, soluble 9 Protein, lgals9.L Protein, lgals9 Protein, lgals9l3 Protein, LGALS9 Protein, Lgals9 Protein

Background: LGALS9,HUAT,LGALS9A

Gene ID: 3965

UniProt: O00182-2