MERS-Coronavirus Spike Protein (RBD) (mFc Tag)

Catalog No. ABIN7535170

Product Details

Target: MERS-Coronavirus Spike (MERS-CoV S) (MERS-Coronavirus Spike Protein (MERS-CoV S))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: RBD

Origin: Middle East Respiratory Syndrome Coronavirus (MERS-CoV)

Source: HEK-293 Cells

Purification tag / Conjugate: This MERS-Coronavirus Spike protein is labelled with mFc Tag.

Purpose: Active Recombinant MERS-CoV Spike RBD Protein

Sequence: EAKPSGSVVE QAEGVECDFS PLLSGTPPQV YNFKRLVFTN CNYNLTKLLS LFSVNDFTCS QISPAAIASN CYSSLILDYF SYPLSMKSDL SVSSAGPISQ FNYKQSFSNP TCLILATVPH NLTTITKPLK YSYINKCSRL LSDDRTEVPQ LVNANQYSPC VSIVPSTVWE DGDYYRKQLS PLEGGGWLVA SGSTVAMTEQ LQMGFGITVQ YGTDTNSVCP KLEFANDTKI ASQLGNCVEY

Specificity: Glu367-Tyr606

Purity: > 90 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human CD26 at 2μg/mL (100μL/well) can bind MERS-CoV Spike RBD with a linear range of 0.5-139.82ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: MERS-Coronavirus Spike (MERS-CoV S) (MERS-Coronavirus Spike Protein (MERS-CoV S))

Alternative Name: MERS-CoV Spike (MERS-CoV S Products)

Target Type: Viral Protein

Background:

Description: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Name: MERS-CoV Spike RBD

Gene ID: 14254594

UniProt: K9N5Q8