ERK2 Protein (GST tag)

Catalog No. ABIN7520337

Product Details

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ERK2 protein is labelled with GST tag.

Purpose: Active Recombinant Human ERK2/MAPK1 Protein

Sequence: MAAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR VAIKKISPFE HQTYCQRTLR EIKILLRFRH ENIIGINDII RAPTIEQMKD VYIVQDLMET DLYKLLKTQH LSNDHICYFL YQILRGLKYI HSANVLHRDL KPSNLLLNTT CDLKICDFGL ARVADPDHDH TGFLTEYVAT RWYRAPEIML NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI LGILGSPSQE DLNCIINLKA RNYLLSLPHK NKVPWNRLFP NADSKALDLL DKMLTFNPHK RIEVEQALAH PYLEQYYDPS DEPIAEAPFK FDMELDDLPK EKLKELIFEE TARFQPGYRS

Specificity: Met1-Ser360

Purity: > 70 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM AEBSF, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: ERK2/MAPK1 (MAPK1 Products)

Synonyms: ERK-1 Protein, ERK1 Protein, ERT2 Protein, HS44KDAP Protein, HUMKER1A Protein, P44ERK1 Protein, P44MAPK Protein, PRKM3 Protein, p44-ERK1 Protein, p44-MAPK Protein, Erk-1 Protein, Erk1 Protein, Ert2 Protein, Esrk1 Protein, Mnk1 Protein, Mtap2k Protein, Prkm3 Protein, p44 Protein, p44erk1 Protein, p44mapk Protein, ERK Protein, ERK2 Protein, ERT1 Protein, MAPK2 Protein, P42MAPK Protein, PRKM1 Protein, PRKM2 Protein, p38 Protein, p40 Protein, p41 Protein, p41mapk Protein, 9030612K14Rik Protein, AA407128 Protein, AU018647 Protein, C78273 Protein, Erk2 Protein, Prkm1 Protein, p42mapk Protein, zERK2 Protein, MAPK1 Protein, Xp42 Protein, erk Protein, erk2 Protein, ert1 Protein, mapk Protein, mapk1b Protein, mapk2 Protein, mpk1 Protein, prkm1 Protein, prkm2 Protein, mapk1 Protein, mapk1a Protein, xp42 Protein, ATMPK1 Protein, F14N23.9 Protein, F14N23_9 Protein, MITOGEN-ACTIVATED PROTEIN KINASE Protein, mitogen-activated protein kinase 1 Protein, MNK1 Protein, ATMPK2 Protein, MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2 Protein, T30E16.13 Protein, T30E16_13 Protein, mitogen-activated protein kinase homolog 2 Protein, mitogen-activated protein kinase 3 Protein, mitogen-activated protein kinase 1 Protein, mitogen activated protein kinase 1 Protein, mitogen-activated protein kinase 1 L homeolog Protein, mitogen-activated protein kinase 1 S homeolog Protein, mitogen activated protein kinase 3 Protein, mitogen-activated protein kinase homolog 2 Protein, MAPK3 Protein, Mapk3 Protein, MAPK1 Protein, Mapk1 Protein, mapk1 Protein, mapk1.L Protein, mapk1.S Protein, PfMAP1 Protein, MPK1 Protein, PVX_084965 Protein, LOC9328617 Protein, Tsp_01601 Protein, MPK2 Protein

Background:

Description: ERK2 is a protein serine/threonine kinase, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions.

Name: ERK,ERK-2,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk,p42-MAPK,MAPK1

Gene ID: 5594

UniProt: P28482

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins