TFF1 Protein (pSer2) (His tag)

Catalog No. ABIN7536423

Product Details

Target: TFF1 (Trefoil Factor 1 (TFF1))

Protein Type: Recombinant

Protein Characteristics: pSer2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TFF1 protein is labelled with His tag.

Purpose: Recombinant Human BCEI/PS2/TFF1 Protein

Sequence: EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEECEF

Specificity: Glu25-Phe84

Purity: > 97 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.001 EU/μg

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Concentration: 1.1 mg/mL

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: TFF1 (Trefoil Factor 1 (TFF1))

Alternative Name: BCEI//TFF1 (TFF1 Products)

Synonyms: BCEI Protein, D21S21 Protein, HP1.A Protein, HPS2 Protein, pNR-2 Protein, pS2 Protein, Bcei Protein, PS2 Protein, bcei Protein, d21s21 Protein, hp1.a Protein, hps2 Protein, p1 Protein, p1-a Protein, pnr-2 Protein, ps2 Protein, tff1 Protein, xP1 Protein, xp1-L Protein, xp1 Protein, TFF1 Protein, Ps2 Protein, trefoil factor 1 Protein, trefoil factor 1 S homeolog Protein, trefoil factor 1, gene 2 S homeolog Protein, TFF1 Protein, Tff1 Protein, tff1.S Protein, tff1.2.S Protein, LOC100358238 Protein

Background:

Description: Trefoil Factor 1 (TFF1), also known as pS2, is one of three structurally related secreted proteins that contain trefoil domains. These domains adopt a three-leaved conformation held together by conserved intrachain disulfide bonds. TFF1 is an approximately 7 kDa peptide that plays an important role in epithelial regeneration and wound healing (1). Mature human TFF1 shares 67 % amino acid sequence identity with mouse and rat TFF1. It is expressed by goblet cells of the gastric and intestinal mucosa and by conjunctival goblet cells (2-5). TFF1 is a copper-binding protein that can form disulfide-linked homodimers, associate into disulfide-linked complexes with Gastrokine 2, and form non-covalent complexes with the mucin MUC5AC (4, 6-8). Copper enhances TFF1 homodimerization as well as its ability to promote epithelial cell motility, wound healing, and the colonization of H. pylori in stomach and colon epithelia (9, 10). TFF1 is down-regulated during the progression from gastritis to gastric dysplasia to gastric cancer, although it is up-regulated in breast and prostate cancers (11-13). TFF1 inhibits the formation of calcium oxalate crystals, and its excretion in the urine is reduced in patients with kidney stones (14).

Name: pS2, BCEI, HPS2, HP1.A, pNR-2, D21S21,TFF1

Gene ID: 7031

UniProt: P04155

Pathways: EGFR Signaling Pathway