FCGR1A Protein (AA 16-281) (His-Avi Tag)

Catalog No. ABIN7536863

Product Details

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Protein Type: Recombinant

Protein Characteristics: AA 16-281

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FCGR1A protein is labelled with His-Avi Tag.

Application: SDS-PAGE (SDS), Size-exclusion chromatography-High Pressure Liquid Chromatography (SEC-HPLC), Surface Plasmon Resonance (SPR)

Purpose: Human Fc gamma RI / CD64 protein

Sequence: QVDTTKAVIT LQPPWVSVFQ EETVTLHCEV LHLPGSSSTQ WFLNGTATQT STPSYRITSA SVNDSGEYRC QRGLSGRSDP IQLEIHRGWL LLQVSSRVFT EGEPLALRCH AWKDKLVYNV LYYRNGKAFK FFHWNSNLTI LKTNISHNGT YHCSGMGKHR YTSAGISVTV KELFPAPVLN ASVTSPLLEG NLVTLSCETK LLLQRPGLQL YFSFYMGSKT LRGRNTSSEY QILTARREDS GLYWCEAATE DGNVLKRSPE LELQVLGGGL NDIFEAQKIE WHEGGGENLY FQSGGHHHHH HHHHH

Specificity: IgG

Characteristics: The sequence of the extracellular domain of human CD64 (Gln 16-Leu 281) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.

Purification: Nickel and SEC

Purity: > 95 % by SEC-HPLC

Endotoxin Level: <1.0 EU per mg

Biological Activity Comment: Measured by its binding affinity in a SPR assay on a Biacore 8k instrument. Human Fc gamma RI / CD64 protein can bind to anti-HER2 human IgG1 (trastuzumab) immobilized on a Protein A chip with an affinity constant (KD) of 0.1 nM.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: To obtain a final concentration of 1 mg/mL reconstitute 250 μg vials with 250 μL water and 1.0 mg vials with 1.0 mL water. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Do not vortex.

Concentration: 1 mg/mL

Buffer: PBS pH 7.2-7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4)

Preservative: Without preservative

Storage: RT,4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.

Expiry Date: 12 months

Target Details

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Alternative Name: CD64 (FCGR1A Products)

Synonyms: CD64 Protein, CD64A Protein, FCRI Protein, IGFR1 Protein, FcgammaRI Protein, Fcgr1 Protein, Fcgr1b Protein, AI323638 Protein, AV092959 Protein, IGGHAFC Protein, FCGR1A Protein, Fc fragment of IgG receptor Ia Protein, Fc receptor, IgG, high affinity I Protein, FCGR1A Protein, Fcgr1a Protein, Fcgr1 Protein

Background: CD64, CD64A, FCGR1, FCGRI, FCGR1A, FCGRIA, FCR1, FCRI, IGFR1
Background: IGFRI,High affinity immunoglobulin gamma Fc receptor I, also known as FcγRI or CD64, is a type I integral membrane glycoprotein. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes, macrophages, dendritic cells and activated granulocytes. CD64 binds with high affinity to the Fc domain of IgG and it plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC). CD64 is structurally composed of three extracellular immunoglobulin domains of the C2-type that interact with the IgG Fc domain, a transmembrane domain and a short cytoplasmic tail. CD64 is associated with a dimer of the common Fc receptor gamma-chain which contains the immunoreceptor tyrosine-based activation (ITAM) motif. The product provided only contains the extracellular portion of CD64.

Molecular Weight: 34.4 kDa

UniProt: P12314

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process