CD25 Protein (AA 22-236) (His tag)
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- Target See all CD25 (IL2RA) Proteins
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 22-236
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Origin
- Mouse
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Source
- Mammalian Cells
- Purification tag / Conjugate
- This CD25 protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), Immunogen (Imm), ELISA, Activity Assay (AcA)
- Purpose
- Recombinant Mouse CD25/IL2RA Protein, C-10*His (Active)
- Purity
- >90 % as determined by SDS-PAGE.
- Endotoxin Level
- <0.1 EU/μg of the protein by the LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit the IL-2-dependent proliferation of human megakaryocytic leukemic cells (MO7e). The ED50 for this effect is 0.045-0.3 μg/mL in the presence of 30 ng/mL of recombinant human IL-2.
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Interleukin 2 Receptor, alpha (IL2RA) (AA 22-236) protein (His tag)
IL2RA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) protein (His-Avi Tag)IL2RA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) protein (His-Avi Tag,Biotin)IL2RA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) protein (His tag)IL2RA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) (Active) protein (Fc Tag)IL2RA Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-236) (Active) protein (His tag)IL2RA Origin: Mouse Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-240) protein (hIgG-His-tag)IL2RA Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-236) protein (Fc Tag)IL2RA Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) (Active) protein (His tag,AVI tag,Biotin)IL2RA Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
- Expiry Date
- 12 months
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- Target
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Alternative Name
- Interleukin-2 receptor subunit alpha (IL2RA Products)
- Background
- Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, CD25, IL2RA
- UniProt
- Q544I2
- Pathways
- JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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