CD25 Protein (AA 22-213) (His-Avi Tag)

Catalog No. ABIN7274199

This Recombinant CD25 protein is produced in HEK-293 Cells.

Quick Overview for CD25 Protein (AA 22-213) (His-Avi Tag) (ABIN7274199)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-213

Purification tag / Conjugate: This CD25 protein is labelled with His-Avi Tag.

Purpose: Human IL-2 R alpha/CD25 Protein

Sequence: Glu22-Cys213

Characteristics: Recombinant Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu22-Cys213.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-2 R alpha, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 15.1ng/ml determined by ELISA. The affinity constant of 15.2nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: IL-2 R alpha

Background: Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL-2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Molecular Weight: 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation