CD25 Protein (AA 22-213) (His tag)

Catalog No. ABIN2018091

Product Details

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 22-213

Origin: Human

Source: HEK-293T Cells

Purification tag / Conjugate: This CD25 protein is labelled with His tag.

Characteristics: Determined by its ability to increase the proliferation effect of IL-2 in murine CTLL-2 cells. In the presence of 1 ng/mL of recombinant IL-2, ED50 for this effect is < 1.5 μg/mL.
AA 22-213, expressed with N-terminal 8xHis

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant Human Interleukin-2 Receptor alpha remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, Human Interleukin-2 Receptor alpha should be stable up to 1 week at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: Interleukin-2 Receptor Alpha(IL-2 R Alpha) (IL2RA Products)

Synonyms: CD25 Protein, IDDM10 Protein, IL2R Protein, TCGFR Protein, IL2RAC Protein, IL-2RA Protein, Il2r Protein, Ly-43 Protein, interleukin 2 receptor subunit alpha Protein, interleukin-2 receptor subunit alpha-like Protein, interleukin 2 receptor, alpha chain Protein, IL2RA Protein, Il2ra Protein, LOC100222957 Protein

Background: Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to the cytokine IL-2. The IL-2R is made up of 3 subunits - alpha , beta and gamma . The alpha and beta chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the gamma-chain, along with the beta subunit. The beta and gamma chains of the IL-2R are members of the type I cytokine receptor family. IL-2R has a high binding affinity to IL-2 and is expressed by antigen-activated T lymphocytes (T cells). IL-2 R alpha is also known as CD25, p55, and Tac (activated T cell) antigen.Recombinant Human Interleukin-2 Receptor alpha (IL-2 R alpha), His produced in HEK 293 cells is a polypeptide chain containing 205 amino acids. A fully biologically active molecule, rhIL-2 R alpha has a molecular mass of 42 kDa analyzed by reducing SDS-PAGE.
Synonyms: soluble IL-2 receptor, TAC-antigen, CD25 antigen

Molecular Weight: 42 kDa, observed by reducing SDS-PAGE.

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation