CD25 Protein (AA 22-240) (hIgG-His-tag)
Quick Overview for CD25 Protein (AA 22-240) (hIgG-His-tag) (ABIN5854928)
Target
See all CD25 (IL2RA) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 22-240
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Purification tag / Conjugate
- This CD25 protein is labelled with hIgG-His-tag.
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Sequence
- ADPELCDDDP PEIPHATFKA MAYKEGTMLN CECKRGFRRI KSGSLYMLCT GNSSHSSWDN QCQCTSSATR NTTKQVTPQP EEQKERKTTE MQSPMQPVDQ ASLPGHCREP PPWENEATER IYHFVVGQMV YYQCVQGYRA LHRGPAESVC KMTHGKTRWT QPQLICTGEM ETSQFPGEEK PQASPEGRPE SETSCLVTTT DFQIQTEMAA TMETSIFTTE YQLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H
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Purification
- > 90% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) protein (His-Avi Tag)
IL2RA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) (Active) protein (Biotin,His-Avi Tag)IL2RA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) protein (His tag)IL2RA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) (Active) protein (Fc Tag)IL2RA Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-236) protein (Fc Tag)IL2RA Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Interleukin 2 Receptor, alpha (IL2RA) protein (His tag)IL2RA Origin: Mouse Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE
Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) (Active) protein (His tag,AVI tag,Biotin)IL2RA Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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Alternative Name
- IL-2R alpha/IL2RA
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Background
- IL2RA, also known as interleukin-2 receptor subunit alpha isoform 1, is a type 1 transmembrane glycoprotein. This protein is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, mastocytosis and tumor infiltrating lymphocytes. It functions as the receptor for HTLV-1 and is consequently expressed on neoplastic cells in adult T cell lymphoma/leukemia. Also, it is elevated in proliferative disturbances of the hematopoietic system and in many solid tumors. Recombinant human IL2RA, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 52.1 kDa (461aa)
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NCBI Accession
- NP_000408
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Pathways
- JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
Target
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