Leptin Protein (LEP) (AA 22-167)

Catalog No. ABIN7664473

This Recombinant Leptin protein is produced in Escherichia coli (E. coli).

Quick Overview for Leptin Protein (LEP) (AA 22-167) (ABIN7664473)

Target: Leptin (LEP)

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 22-167

Purpose: Recombinant Mouse Leptin

Endotoxin Level: < 1 EU/μg as determined by LAL test.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 300 mM NaCl, pH 7.5.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20°C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80°C for 3 months or 1 week at 2 to 8°C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: Leptin (LEP)

Alternative Name: Leptin

Background: Leptin, Obese Protein, Obesity Factor, LEP, OB, OBS

Molecular Weight: 14 kDa

UniProt: Q544U0

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process