p53 Protein (His tag)
Quick Overview for p53 Protein (His tag) (ABIN7669927)
Target
See all p53 (TP53) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This p53 protein is labelled with His tag.
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Purpose
- Recombinant Mouse BBL protein(His Tag)
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Sequence
- Arg 104-Glu 309
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Purification
- > 95 % as determined by reducing SDS-PAGE.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 0.1 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measure by its ability to induce IL-2 secretion in mouse T cells in the presence of the anti-CD3 antibody.The ED50 for this effect is <0.05 μg/mL.
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Tumor Protein P53 (TP53) (AA 1-393) protein (His tag)
custom-made TP53 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Protein P53 (TP53) (AA 1-393) protein (GST tag)TP53 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Protein P53 (TP53) (Active) protein (His tag)TP53 Origin: Human Host: Escherichia coli (E. coli) Recombinant STD, PI, in vitro Active
Tumor Protein P53 (TP53) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)TP53 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining STD, AbP
Tumor Protein P53 (TP53) (AA 1-391) protein (His tag)TP53 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Tumor Protein P53 (TP53) protein (His-SUMO Tag)TP53 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90% as determined by SDS-PAGE. ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- p53 (TP53) (Tumor Protein P53 (TP53))
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Alternative Name
- BBL
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Background
- Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.,BAF,BL,BLyS,D8Ertd387,D8Ertd387e,TAL,TALL-1,TALL1,THANK,TNFSF20,Tnfsf13b,Tnlg7a,member 13b,tumor necrosis factor (ligand) superfamily,zTNF,zTNF4
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Molecular Weight
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calculated_mw: 23.9 kDa
observed_mw: 24 kDa
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UniProt
- P41274
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Pathways
- p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
Target
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