HRAS Protein (G12C) (His-TEV cleavage gag)

Catalog No. ABIN7856454

This Recombinant HRAS protein is produced in Escherichia coli (E. coli).

Quick Overview for HRAS Protein (G12C) (His-TEV cleavage gag) (ABIN7856454)

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Product Details

Protein Characteristics: G12C

Purification tag / Conjugate: This HRAS protein is labelled with His-TEV cleavage gag.

Purpose: Recombinant HRAS (G12C) Protein

Sequence: 2-186

Characteristics: Recombinant human HRAS (G12C) (2-186) was expressed in E. coli cells using an N-terminal His-TEV tag. HRAS (G12C) is constitutively active.

Sterility: Sterile

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile 50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 50-200 mM imidazole, 0-0.1 mM PMSF, 0.2-1.0 mM DTT, 10-25 % glycerol

Preservative: Dithiothreitol (DTT), PMSF

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

Expiry Date: 12 months

Target Details

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name: HRAS

Molecular Weight: ~23 kDa

NCBI Accession: NM_005343

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling