HMGB1 Protein (Terminally oxidized)

Catalog No. ABIN7866190

This Recombinant HMGB1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for HMGB1 Protein (Terminally oxidized) (ABIN7866190)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Human, Mouse, Rat

Source: Escherichia coli (E. coli)

Application: Activation (Act), Cellular Assay (CA)

Product Details

Protein Characteristics: Terminally oxidized

Purpose: Terminally oxidized HMGB1, LPS-Free

Sequence: Terminally oxidized-HMGB1 consists of 215 amino acid: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE

Characteristics: HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells (Dumitriu et al. 2005). HMGB1 is essential for life: Hmgb1 knockout mice die shortly after birth.
The two different activities of HMGB1 as a chemoattractanct of motile cells and as inducer of cytokines have been attributed to different biochemical forms. Fully reduced HMGB1 (the form with chemoattractanct activity) is completely reduced; disulfide HMGB1 contains a disulfide bond between C23 and C45 (complete notation: HMGB1C23-C45C106h – Antoine J. et al 2014).
Terminally oxidized-HMGB1 has all the cysteines oxidized to sulfonates and has no activity, either as a chemoattractant or in cytokine stimulation (Kazama et al,2008 ;Yang et al, 2012).

Purification: Purified

Endotoxin Level: LPS-free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL

Biological Activity Comment: Terminally oxidized-HMGB1 has all the cysteines oxidized to sulfonates and has no activity, either as a chemoattractant or in cytokine stimulation

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl.

Buffer: The protein is lyophilized from 50 mM HEPES buffer, pH 7.9 and 500 mM NaCl.
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request.

Storage: 4 °C/-20 °C

Storage Comment: 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C).

Target Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: High-mobility group protein B1 (HMGB1)

Background: HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.

Molecular Weight: 24.8 kDa

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome