HMGB1 Protein (BoxA 2D)
Quick Overview for HMGB1 Protein (BoxA 2D) (ABIN7866199)
Target
See all HMGB1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- BoxA 2D
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Purpose
- BoxA 2D (negative control of BoxA wt), LPS-free
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Sequence
- BoxA 2D from HMGB1 consists of 89 amino acids: MGKGDPKKPR GKMSSYAFFV QTDREEHKKK HPDASVNFSE FSKKDSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKF
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Characteristics
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BoxA 2D is the mutant form of BoxA (DNA binding domains of HMGB1 protein) in which the two cysteines are replaced with aspartic acid.
It consists of 89 amino acids and has a calculated molecular mass of approximately 10.4 kDa.
BoxA 2D in migration assay cannot block the HMGB1 induced migration of fibroblasts.
It contains only trace amounts of LPS (<0.4 ng/mg protein).
BoxA 2D variant can be used as negative control of BoxA wt. -
Purification
- Purified
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Endotoxin Level
- LPS-free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL
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High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (AA 1-215) (Active) protein (Biotin,His tag)HMGB1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
High Mobility Group Box 1 (HMGB1) (Fully reduced) (Active) proteinHMGB1 Origin: Human, Mouse, Rat Host: Escherichia coli (E. coli) Recombinant > 95 % It contains no nucleic acids. Act, CA Active
High Mobility Group Box 1 (HMGB1) (C106S), (C23S), (C45S), (Non-oxidizable) (Active) proteinHMGB1 Origin: Human, Mouse, Rat Host: Escherichia coli (E. coli) Recombinant > 95 % It contains no nucleic acids. Act, CA Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) proteinHMGB1 Origin: Human Host: Baculovirus Recombinant > 90 % pure SDS
High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)HMGB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)HMGB1 Origin: Mouse Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- The lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl.
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Buffer
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The protein is lyophilized from 50 mM HEPES buffer, pH 7.9 and 500 mM NaCl.
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request. -
Storage
- 4 °C/-20 °C
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Storage Comment
- 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C).
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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Alternative Name
- High-mobility group protein B1 (HMGB1)
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Background
- HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.
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Molecular Weight
- 10.4 kDa
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Pathways
- p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
Target
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