IL-1 beta Protein
Quick Overview for IL-1 beta Protein (ABIN7956227)
Target
See all IL-1 beta (IL1B) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purpose
- Recombinant Rat Interleukin-1 beta
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Sequence
- MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 1 EU/ug of rRtIL-1β as determined by LAL method.
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Biological Activity Comment
- Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x107 IU/mg.
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Interleukin 1, beta (IL1B) (AA 117-269) protein (His-Avi Tag)
IL1B Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Interleukin 1, beta (IL1B) (AA 117-269) (Active) protein (Biotin,His-Avi Tag)IL1B Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 1, beta (IL1B) (AA 1-269) protein (GST tag)IL1B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 1, beta (IL1B) (AA 117-269) protein (Fc Tag)IL1B Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Interleukin 1, beta (IL1B) (AA 170-269) protein (GST tag)IL1B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 1, beta (IL1B) (AA 117-269) (Active) protein (His tag)IL1B Origin: Human Host: Escherichia coli (E. coli) Recombinant >90 % as determined by SDS-PAGE. WB, SDS, Imm, ELISA, AcA Active
Interleukin 1, beta (IL1B) (AA 118-269) (Active) protein (His tag)IL1B Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >90 % as determined by SDS-PAGE. WB, SDS, Imm, ELISA, AcA Active
Interleukin 1, beta (IL1B) (Active) proteinIL1B Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 97 % as determined by SDS-PAGE Active
Interleukin 1, beta (IL1B) (Active) proteinIL1B Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 98 % as determined by SDS-PAGE Active
Interleukin 1, beta (IL1B) (AA 106-267) protein (His tag)IL1B Origin: Chicken Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Comment
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Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Expiry Date
- 12 months
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- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
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Alternative Name
- Interleukin-1 beta
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Molecular Weight
- 17.3 kDa
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UniProt
- Q63264
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Pathways
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
Target
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