HMGB1 Protein (AA 9-163)
Quick Overview for HMGB1 Protein (AA 9-163) (ABIN8128032)
Target
See all HMGB1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 9-163
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Purpose
- Active High Mobility Group Protein 1 (HMGB1)
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Sequence
- Pro9-Arg163
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High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
High Mobility Group Box 1 (HMGB1) (Fully reduced) (Active) proteinHMGB1 Origin: Human, Mouse, Rat Host: Escherichia coli (E. coli) Recombinant > 95 % It contains no nucleic acids. Act, CA Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) (Active) protein (Biotin,His tag)HMGB1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
High Mobility Group Box 1 (HMGB1) (BoxA) (Active) proteinHMGB1 Origin: Human, Mouse Host: Escherichia coli (E. coli) Recombinant > 98 % It contains no nucleic acids. Act, CA Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS
High Mobility Group Box 1 (HMGB1) (AA 1-215) proteinHMGB1 Origin: Human Host: Baculovirus Recombinant > 90 % pure SDS
High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)HMGB1 Origin: Mouse Host: HEK-293 Cells Recombinant > 85 % as determined by reducing SDS-PAGE. ELISA Active
High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)HMGB1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 9.7
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 20 mM Tris, 150 mM NaCl ( pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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Alternative Name
- High Mobility Group Protein 1
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Background
- HMG1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein
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Molecular Weight
- 22kDa
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UniProt
- P09429
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Pathways
- p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
Target
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