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Inhibin, beta B (INHBB) (Active) protein (His tag) Protein

INHBB Origin: Human Source: Tobacco (Nicotiana benthamiana) Recombinant > 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. CC, WB
Catalog No. ABIN809201
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  • Protein Name
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    Human
    Source
    Tobacco (Nicotiana benthamiana)
    Purification tag / Conjugate
    This INHBB protein is labelled with His tag.
    Application
    Cell Culture (CC), Western Blotting (WB)
    Characteristics
    Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody Activin B.
    Molecular formula: C615H910N178O177S12.
    Isoelectric Point: 6,4.
    Extinction coefficient: E 0.1 % (1g/L) = 1.56 (A 280 nm).
    This product contains no animal-derived components or impurities. Produced by transient expression of Activin B in non-transgenic plants.
    Purification
    Recombinant human Activin B contains a His-tag at the N-terminal end and is purified by sequential chromatography (FPLC).
    Purity
    > 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
    Endotoxin Level
    < 0.04 EU/µg protein (LAL method)
  • Comment

    Biological Activity: The biological activity of Activin B is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. Less than 5 ng/mL are required to stimulate a half-maximal response at cytokine saturation. Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Lyophilized protein should be reconstituted in water following instructions of batch Quality Control sheet. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.
    Buffer
    Tris HCl 0.05 M buffer at pH 7.4
    Storage
    4 °C
  • Protein Name
    Background
    Synonyms: Activin beta-B chain, Inhibin beta B chain precursor, Inhibin, beta-2
    Activins are homodimers or heterodimers of the various beta subunit isoforms, belonging to the TGF-beta family. Mature Activin B has two chains of 123 amino acids residues (betaB-betaB). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Inhibins/Activins are protein that are formed by the dimerization of two subunits, i. e. an alpha (alpha) with either beta A (betaA) - Inhibin A or beta B (betaB) - Inhibin B. The subunits betaA and betaB can also form homodimers or heterodimers called Activin: Activin A (betaA -betaA), Activin B (betaB -betaB) and Activin AB (betaA -betaB). The Activin gene family comprises the additional, but poorly characterized members’ Activin betaC (betaC), beta D (betaD) and beta E (betaE). As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B.
    Molecular Weight
    14 kDa
    Gene ID
    3625, 147390
    Pathways
    Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion
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