IL12 Protein

Catalog No. ABIN935473

Product Details

Target: IL12 (Interleukin 12 (IL12))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Sequence: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS

Characteristics: Purified recombinant Human IL12 protein
Expression System: CHO cells
Bioactivity: The ED50 was determined by the induction of IFN-gamma from NK cells co-stimulated with IL-18 is ? 1.0 ng/mL, corresponding to a specific activity of ? 1 x 10^6 units/mg.

Purity: > 98 % pure

Endotoxin Level: < 0.1 ng per µg (1 EU/µg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 1 xPBS to a concentration of 0.2 mg/mL.

Buffer: Lyophilized from 0.5 x PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Target Details

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL12 (IL12 Products)

Synonyms: CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, LOC100217488 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p40 Protein, IL12B Protein, IL-12 p35 Protein, interleukin 12B Protein, interleukin 12A Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12B Protein, IL12A Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein

Background: IL12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities IL12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-gamma producing Th1 cells.
Alternative Names: NKSF protein, IL-12, Cytotoxic lymphocyte maturation factor protein, IL-12 protein, TSF protein, IL 12, CLMF protein, Interleukin 12 protein, IL 12 protein, CTL maturation factor protein, TCMF protein, IL-12 protein, IL12

Molecular Weight: 75 kDa

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome