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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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4.751972 | ABIN2777194 | WB | Rabbit | N-Term | Log in to see | Polyclonal | 0 | |||
4.751972 | ABIN2774828 | WB | Rabbit | Middle Region | Log in to see | Polyclonal | 0 |
General |
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Antigen | Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) Antibodies |
Reactivity | Amphibian, Human, Mouse (Murine), Primate, Rat (Rattus), Zebrafish (Danio rerio) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This ARNT antibody is un-conjugated Alternatives |
Application |
Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
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11 references available |
Supplier | Log in to see |
Product Details anti-ARNT AntibodyTarget Details ARNT Application Details Handling References for anti-ARNT antibody (ABIN152684) Images |
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Specificity | Detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT). |
Purification | Immunogen affinity purified |
Immunogen | Synthetic peptide corresponding to residues C N(771) S Y N N E E F P D L T M F P P F S E(789) of human ARNT. |
Clone | 2B10 |
Isotype | IgG1 |
Plasmids, Primers & others | |
Target Details ARNTProduct Details anti-ARNT Antibody Application Details Handling References for anti-ARNT antibody (ABIN152684) Images back to top |
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Antigen | |
Alternative Name | ARNT/HIF-1 beta (ARNT Antibody Abstract) |
Background | Gene Symbol: ARNT |
Gene ID | 405 |
UniProt | P27540 |
Pathways | Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2 |
Application DetailsProduct Details anti-ARNT Antibody Target Details ARNT Handling References for anti-ARNT antibody (ABIN152684) Images back to top |
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Application Notes | Western Blot 1:500 - 1:3000, Immunohistochemistry 5 μg/mL, Immunocytochemistry/Immunofluorescence 1:1000, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 5 μg/mL, Gel Super Shift Assays 1:1 - 1:100May be useful in ELISA, IF, IP, Gel Super Shift Assays, and WB. ChIP usage was reported in scientific literature. WB: Detects an approx. 87 kDa protein representing ARNT in COS cell extract. IF: Staining of ARNT in VT{2} cells (Hepa 1-C4T mutant deficient in ARNT function but stably transfected with the full length human ARNT cDNA) using NB 300-525 yields non-nucleolar, nuclear staining only. |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
HandlingProduct Details anti-ARNT Antibody Target Details ARNT Application Details References for anti-ARNT antibody (ABIN152684) Images back to top |
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Format | Liquid |
Concentration | 1 mg/mL |
Buffer |
PBS with 1.0 mg/mL BSA Buffer contains: 0.05 % Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid freeze-thaw cycles |
Storage | -20 °C |
Storage Comment | Store at -20°C. Avoid freeze-thaw cycles. |
References for anti-ARNT antibody (ABIN152684)Product Details anti-ARNT Antibody Target Details ARNT Application Details Handling Images back to top |
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Product cited in: |
Mandl, Lieberum, Depping: "A HIF-1α-driven feed-forward loop augments HIF signalling in Hep3B cells by upregulation of ARNT." in: Cell death & disease, Vol. 7, Issue 6, pp. e2284, 2016 Method employed by authors: Western Blotting (WB) Mandl, Lieberum, Dunst, Depping: "The expression level of the transcription factor Aryl hydrocarbon receptor nuclear translocator (ARNT) determines cellular survival after radiation treatment." in: Radiation oncology (London, England), Vol. 10, pp. 229, 2015 (Sample species: Human). Further details: Western Blotting Thomas, Harding, Smith, Overdevest, Nitz, Frierson, Tomlins, Kristiansen, Theodorescu: "CD24 is an effector of HIF-1-driven primary tumor growth and metastasis." in: Cancer research, Vol. 72, Issue 21, pp. 5600-12, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation Rahimi, George, Tang: "EGFR variant-mediated invasion by enhanced CXCR4 expression through transcriptional and post-translational mechanisms." in: International journal of cancer, Vol. 126, Issue 8, pp. 1850-1860, 2010 Singh, Wyman, Casado, Garrett, Gasiewicz: "Treatment of mice with the Ah receptor agonist and human carcinogen dioxin results in altered numbers and function of hematopoietic stem cells." in: Carcinogenesis, Vol. 30, Issue 1, pp. 11-9, 2009 Amir, Wang, Simons, Mabjeesh: "SEPT9_v1 up-regulates hypoxia-inducible factor 1 by preventing its RACK1-mediated degradation." in: The Journal of biological chemistry, Vol. 284, Issue 17, pp. 11142-51, 2009 Hord, Perdew et al.: "Physicochemical and immunocytochemical analysis of the aryl hydrocarbon receptor nuclear translocator: characterization of two monoclonal antibodies to the aryl hydrocarbon receptor nuclear ..." in: Molecular pharmacology, Vol. 46, Issue 4, pp. 618-26, 1994 Mandl, Depping: "ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells." in: Cancer cell international, Vol. 17, pp. 77, 1970 Method employed by authors: Western Blotting (WB) (Sample species: Human). |
Background publications |
Hirose, Morita, Ema, Mimura, Hamada, Fujii, Saijo, Gotoh, Sogawa, Fujii-Kuriyama: "cDNA cloning and tissue-specific expression of a novel basic helix-loop-helix/PAS factor (Arnt2) with close sequence similarity to the aryl hydrocarbon receptor nuclear translocator (Arnt)." in: Molecular and cellular biology, Vol. 16, Issue 4, pp. 1706-13, 1996 Vaziri, Schneider, Sherr, Faller: "Expression of the aryl hydrocarbon receptor is regulated by serum and mitogenic growth factors in murine 3T3 fibroblasts." in: The Journal of biological chemistry, Vol. 271, Issue 42, pp. 25921-7, 1996 Hoffman, Reyes, Chu, Sander, Conley, Brooks, Hankinson: "Cloning of a factor required for activity of the Ah (dioxin) receptor." in: Science (New York, N.Y.), Vol. 252, Issue 5008, pp. 954-8, 1991 |
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