anti-Mouse (Murine) BMP2 antibody for Immunohistochemistry

Recommended BMP2 Antibody (supplied by: Log in to see )

Antigen
Bone Morphogenetic Protein 2 (BMP2) Antibodies
  • BDA2
  • BMP2A
  • AI467020
  • Bmp2a
  • BMP-2
  • xBMP-2
  • xbmp2
  • BMP2
  • bmp2a
  • bmp2
  • wu:fc59d09
  • bone morphogenetic protein 2
  • bone morphogenetic protein 2 L homeolog
  • Bone morphogenetic protein 2
  • bone morphogenetic protein 2a
  • BMP2
  • Bmp2
  • bmp2.L
  • bmp2
  • bmp2a
Epitope
Internal Region, AA 250-350
48
24
15
12
12
10
8
7
6
6
5
5
5
5
5
5
5
5
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
237
86
85
28
23
16
9
9
3
3
3
3
2
2
2
1
1
1
Host
Rabbit
230
63
10
Clonality
Polyclonal
Conjugate
This BMP2 antibody is un-conjugated
36
19
8
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
253
139
106
41
29
25
24
24
13
11
9
8
4
3
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN446957
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.854502 ABIN2705600 IHC WB Rabbit Center Log in to see Polyclonal 0
8.854502 ABIN1871311 IHC WB Rabbit IgG Log in to see Polyclonal 0
8.854502 ABIN3183525 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
8.854502 ABIN2879434 IHC IHC (p) WB Rabbit Pro275 Log in to see Polyclonal 0
8.854502 ABIN1100286 ELISA IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
8.854502 ABIN6260279 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
8.854502 ABIN5963013 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4284882 IHC IHC (p) WB Biotin Rabbit AA 250-350, Internal Region Log in to see Polyclonal 1
1 ABIN5663611 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1077864 ICC IHC WB Rabbit IgG AA 281-394 Log in to see Polyclonal 0
1 ABIN2988490 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1452960 IHC ELISA WB Rabbit IgG AA 226-275 Log in to see Polyclonal 0
1 ABIN6260280 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2887771 ELISA IHC IHC (p) WB Rabbit IgG AA 226-275 Log in to see Polyclonal 0
1 ABIN4284881 IHC IHC (p) WB Rabbit AA 250-350, Internal Region Log in to see Polyclonal 0
1 ABIN4284884 IHC IHC (p) WB DyLight 488 Rabbit AA 250-350, Internal Region Log in to see Polyclonal 1
1 ABIN4284883 IHC IHC (p) WB DyLight 650 Rabbit AA 250-350, Internal Region Log in to see Polyclonal 1
1 ABIN2887889 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2617651 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN2746322 CM ICC IF IP IHC ELISA WB FITC Rabbit IgG AA 30-70 Log in to see Polyclonal 0

General

Antigen Bone Morphogenetic Protein 2 (BMP2) Antibodies
Epitope Internal Region, AA 250-350
(48), (24), (15), (12), (12), (10), (8), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(237), (86), (85), (28), (23), (16), (9), (9), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(230), (63), (10)
Clonality
Polyclonal
Conjugate This BMP2 antibody is un-conjugated
(36), (19), (8), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(253), (139), (106), (41), (29), (25), (24), (24), (13), (11), (9), (8), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-BMP2 Antibody

Target Details BMP2 Application Details Handling References for anti-BMP2 antibody (ABIN446957) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal region of human BMP2 (within residues 250-350) [Swiss-Prot# P12643]
Plasmids, Primers & others

Target Details BMP2

Product Details anti-BMP2 Antibody Application Details Handling References for anti-BMP2 antibody (ABIN446957) Images back to top
Antigen
Alternative Name BMP-2 (BMP2 Antibody Abstract)
Background Gene Symbol: BMP2
Molecular Weight Theoretical MW: 44 kDa
Gene ID 650
UniProt P12643, P21274
Pathways Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-BMP2 Antibody Target Details BMP2 Handling References for anti-BMP2 antibody (ABIN446957) Images back to top
Application Notes Western Blot 0.5 μg/mL, Immunohistochemistry 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:200This BMP2 antibody is useful for Immunohistochemistry-Paraffin and Western Blot, where a band is seen ~44 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for BMP2 antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Protocol Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-BMP2 Antibody Target Details BMP2 Application Details References for anti-BMP2 antibody (ABIN446957) Images back to top
Format Liquid
Concentration 1.15 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-BMP2 antibody (ABIN446957)

Product Details anti-BMP2 Antibody Target Details BMP2 Application Details Handling Images back to top
Product cited in:

Scimeca, Piccirilli, Mastrangeli, Rao, Feola, Orlandi, Gasbarra, Bonanno, Tarantino: "Bone Morphogenetic Proteins and myostatin pathways: key mediator of human sarcopenia." in: Journal of translational medicine, Vol. 15, Issue 1, pp. 34, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Cordner, Friend, Mayo, Badgley, Wallmann, Stallings, Young, Miles, Edwards, Bridgewater: "The BMP2 nuclear variant, nBMP2, is expressed in mouse hippocampus and impacts memory." in: Scientific reports, Vol. 7, pp. 46464, 2017 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Gullard, Croney, Wu, Mamaeva, Sohn, Cao, MacDougall: "Reduced Dentin Matrix Protein Expression in Camurati-Engelmann Disease Transgenic Mouse Model." in: Journal of cellular physiology, Vol. 231, Issue 5, pp. 1106-13, 2016 (Sample species: Mouse (Murine)).

Chen, Choesang, Li, Sun, Pham, Bao, Feola, Westerman, Li, Follenzi, Blanc, Rivella, Fleming, Ginzburg: "Increased hepcidin in transferrin-treated thalassemic mice correlates with increased liver BMP2 expression and decreased hepatocyte ERK activation." in: Haematologica, Vol. 101, Issue 3, pp. 297-308, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Scimeca, Bonanno, Piccirilli, Baldi, Mauriello, Orlandi, Tancredi, Gasbarra, Tarantino: "Satellite Cells CD44 Positive Drive Muscle Regeneration in Osteoarthritis Patients." in: Stem cells international, Vol. 2015, pp. 469459, 2015 Further details: Immunohistochemistry

Yeom, Kim, Lee, Zhang, Lee, Hahm, Sohn, Lee: "Effects of laser acupuncture on longitudinal bone growth in adolescent rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 424587, 2013 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Bridgewater, Mayo, Evanson, Whitt, Dean, Yates, Holden, Schmidt, Fox, Dhunghel, Steed, Adam, Nichols, Loganathan, Barrow, Hancock: "A novel bone morphogenetic protein 2 mutant mouse, nBmp2NLS(tm), displays impaired intracellular Ca2+ handling in skeletal muscle." in: BioMed research international, Vol. 2013, pp. 125492, 2013 (Sample species: Mouse (Murine)).

Kahn, Bosch, Levitt, Goldstein: "Effect of sodium nitrate loading on electrolyte transport by the renal tubule." in: The American journal of physiology, Vol. 229, Issue 3, pp. 746-53, 1976 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Images

Product Details anti-BMP2 Antibody Target Details BMP2 Application Details Handling References for anti-BMP2 antibody (ABIN446957) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Bone Morphogenetic Protein 2 (BMP2) (AA 250-350), (Internal Region) antibody (ABIN446957) anti-Bone Morphogenetic Protein 2 (BMP2) (AA 250-350), (Internal Region) antibody
Western Blotting (WB) image for anti-Bone Morphogenetic Protein 2 (BMP2) (AA 250-350), (Internal Region) antibody (ABIN446957) anti-Bone Morphogenetic Protein 2 (BMP2) (AA 250-350), (Internal Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Bone Morphogenetic Protein 2 (BMP2) (AA 250-350), (Internal Region) antibody (ABIN446957) Immunohistochemistry: BMP-2 Antibody [NBP1-19751] - Analysis of BMP2 in mouse heart u...