anti-Rat (Rattus) Lysine (K)-Specific Demethylase 1A antibody for ELISA

Recommended Lysine (K)-Specific Demethylase 1A Antibody (supplied by: Log in to see )

Antigen
Lysine (K)-Specific Demethylase 1A (KDM1A) Antibodies
  • AOF2
  • BHC110
  • KDM1
  • LSD1
  • 1810043O07Rik
  • AA408884
  • Aof2
  • D4Ertd478e
  • Kdm1
  • Lsd1
  • mKIAA0601
  • DKFZp459N2450
  • RGD1562975
  • aof2
  • kdm1
  • wu:fb82e06
  • wu:fc51a02
  • wu:fj36a05
  • wu:fj66b06
  • KDM1A
  • lsd1
  • xLSD1
  • lysine demethylase 1A
  • lysine (K)-specific demethylase 1A
  • lysine (K)-specific demethylase 1a
  • KDM1A
  • Kdm1a
  • kdm1a
Reactivity
Human, Mouse (Murine), Rat (Rattus)
221
121
92
21
20
10
8
6
5
4
3
2
1
1
Host
Rabbit
168
50
5
1
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
189
76
76
54
32
26
22
15
10
10
7
6
6
3
1
1
1
Supplier
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Catalog No. ABIN6262764
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN1030990 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal 0
7 ABIN2432527 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN2434158 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
5.5 ABIN1002761 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 4
5.5 ABIN1002762 IHC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 4
4 ABIN1031442 ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2568302 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN221425 ELISA WB Mouse IgG Log in to see 0
1 ABIN221426 ELISA FACS ICC IF IHC IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN2936377 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2959793 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN2959792 IF IHC ELISA WB Rabbit IgG Center Log in to see Polyclonal 0
1 ABIN1888030 ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN1887596 IHC ELISA WB Rabbit Center Log in to see Polyclonal 0
-2.020279 ABIN4331730 ELISA ICC IF IHC IHC (p) WB Rabbit Center Log in to see Polyclonal 0

General

Antigen Lysine (K)-Specific Demethylase 1A (KDM1A) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(221), (121), (92), (21), (20), (10), (8), (6), (5), (4), (3), (2), (1), (1)
Host Rabbit
(168), (50), (5), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(189), (76), (76), (54), (32), (26), (22), (15), (10), (10), (7), (6), (6), (3), (1), (1), (1)
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Product details

Target Details Application Details Handling Images
Specificity KDM1A Antibody detects endogenous levels of total KDM1A
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Immunogen A synthesized peptide derived from human KDM1A
Isotype IgG

Target Details

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Antigen
Alternative Name KDM1A (KDM1A Antibody Abstract)
Background

Description: Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.

Gene: KDM1A

Molecular Weight 95kDa
Gene ID 23028
UniProt O60341
Pathways Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product details Target Details Handling Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

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Supplier Images
Western Blotting (WB) image for anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody (ABIN6262764) Western blot analysis of extracts of PC12 lysate using KDM1A antibody. The lane on th...
Immunohistochemistry (IHC) image for anti-Lysine (K)-Specific Demethylase 1A (KDM1A) antibody (ABIN6262764) ABIN6276559 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyd...