anti-Human Histone Deacetylase 9 antibody for Western Blotting

Recommended Histone Deacetylase 9 Antibody (supplied by: Log in to see )

Antigen
Histone Deacetylase 9 (HDAC9) Antibodies
  • HD7
  • HD7b
  • HD9
  • HDAC
  • HDAC7
  • HDAC7B
  • HDAC9B
  • HDAC9FL
  • HDRP
  • MITR
  • AV022454
  • D030072B18Rik
  • HD7B
  • Hdac7b
  • Mitr
  • mKIAA0744
  • hdac9
  • zgc:73392
  • TWIST
  • HDAC9
  • DKFZp459E171
  • HDA09
  • HISTONE DEACETYLASE
  • histone deacetylase 9
  • RGD1310748
  • RGD1563092
  • hdac9-A
  • histone deacetylase 9
  • histone deacetylase 9b
  • histone deacetylase 9 S homeolog
  • HDAC9
  • Hdac9
  • hdac9b
  • HDA9
  • hdac9.S
Reactivity
Human, Mouse (Murine), Rat (Rattus)
142
30
23
8
5
4
4
3
2
2
2
2
1
1
1
1
Host
Rabbit
114
28
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
5
4
4
4
Application
Western Blotting (WB)
131
84
63
41
41
25
13
10
3
2
1
1
Options
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Catalog No. ABIN223304
$ 300.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.8412476 ABIN2775579 WB Rabbit Middle Region Log in to see Polyclonal 1
2.8412476 ABIN2775576 ChIP IHC WB Rabbit C-Term Log in to see Polyclonal 4
2.8412476 ABIN2704894 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0
2.8412476 ABIN1533308 IF IHC ELISA WB Rabbit IgG AA 1017-1066 Log in to see Polyclonal 0
2.8412476 ABIN3031237 IF IP IHC ELISA WB Rabbit Ig Fraction AA 2-32 Log in to see Polyclonal 0
2.8412476 ABIN2736885 WB Rabbit IgG Log in to see Polyclonal 0
2.8412476 ABIN4880758 ELISA FACS WB Mouse IgG1 AA 343-569 Log in to see 2B7C4 0
2.8412476 ABIN1868347 ICC IHC WB Rabbit IgG AA 23-343 Log in to see Polyclonal 0
2.8412476 ABIN1843860 IF/ICC IHC WB Rabbit Log in to see Polyclonal 0
2.8412476 ABIN2462632 IHC ELISA WB Rabbit Log in to see Polyclonal 0
2.8412476 ABIN634236 WB Rabbit Middle Region Log in to see Polyclonal 0
2.8412476 ABIN630205 IHC WB Rabbit C-Term Log in to see Polyclonal 0
2.8412476 ABIN2668321 WB Rabbit C-Term Log in to see Polyclonal 0
2.8412476 ABIN3185018 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
2.8412476 ABIN3022054 IHC WB Rabbit IgG Log in to see Polyclonal 0
2.8412476 ABIN523199 IF WB Mouse AA 1-1069, full length Log in to see Polyclonal 0
2.8412476 ABIN3198117 WB Rabbit Log in to see Polyclonal 0
2.8412476 ABIN4316794 IHC WB Mouse IgG1 Log in to see 7G2 0
2.8412476 ABIN4241762 WB Rabbit Log in to see Polyclonal 0
2.8412476 ABIN5542327 ELISA FACS ICC IHC WB Mouse IgG1 AA 343-569 Log in to see 2B7C4 0

General

Antigen Histone Deacetylase 9 (HDAC9) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(142), (30), (23), (8), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(114), (28)
Clonality
Polyclonal
Conjugate Un-conjugated
(5), (5), (5), (4), (4), (4)
Application Western Blotting (WB)
(131), (84), (63), (41), (41), (25), (13), (10), (3), (2), (1), (1)
Pubmed 3 references available
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Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN412772
Specificity The antibody recognizes a ~110-140 kDa of HDAC-9.
A 75 kDa and a 60 kDa bands can also be detected in some tissue lysates.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 25 of human HDAC-9
Isotype IgG

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name HDAC9 (HDAC9 Antibody Abstract)
Background HDAC family members have been divided into two classes. Class I of the HDACs comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93 % identity in amino acid sequence. Class II of the HDACs comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about two-fold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns.
Synonyms: HD9 , Histone deacetylase 9
Gene ID 9734
UniProt Q9UKV0
Pathways Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development

Application Details

Product details Target Details Handling References Images back to top
Application Notes Western blotting (0.5-4 μg/mL), Immunoprecipitation and immunofluorescence. However, the optimal concentrations should be determined individually. The antibody recognizes a ~110-140 kDa of HDAC-9. A 75 kDa and a 60 kDa bands can also be detected in some tissue lysates.
Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References

Product details Target Details Application Details Handling Images back to top
Product cited in:

Kaluza, Kroll, Gesierich, Manavski, Boeckel, Doebele, Zelent, Rössig, Zeiher, Augustin, Urbich, Dimmeler: "Histone deacetylase 9 promotes angiogenesis by targeting the antiangiogenic microRNA-17-92 cluster in endothelial cells." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 33, Issue 3, pp. 533-43, 2013

Yan, Cao, Reilly, Young, Garcia, Mishra: "Histone deacetylase 9 deficiency protects against effector T cell-mediated systemic autoimmunity." in: The Journal of biological chemistry, Vol. 286, Issue 33, pp. 28833-43, 2011

Maltepe, Krampitz, Okazaki, Red-Horse, Mak, Simon, Fisher: "Hypoxia-inducible factor-dependent histone deacetylase activity determines stem cell fate in the placenta." in: Development (Cambridge, England), Vol. 132, Issue 15, pp. 3393-403, 2005

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Histone Deacetylase 9 (HDAC9) antibody (ABIN223304) Western blot analysis of HDAC-9 in mouse small intestine tissue lysate.