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|Antigen||Histone Deacetylase 9 (HDAC9) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Western Blotting (WB)
|3 references available|
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|Blocking Peptide||Blocking peptide for this product available: ABIN412772|
The antibody recognizes a ~110-140 kDa of HDAC-9.
A 75 kDa and a 60 kDa bands can also be detected in some tissue lysates.
|Immunogen||Synthetic peptide surrounding amino acid 25 of human HDAC-9|
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|Alternative Name||HDAC9 (HDAC9 Antibody Abstract)|
HDAC family members have been divided into two classes. Class I of the HDACs comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93 % identity in amino acid sequence. Class II of the HDACs comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about two-fold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns.
Synonyms: HD9 , Histone deacetylase 9
|Pathways||Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development|
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|Application Notes||Western blotting (0.5-4 μg/mL), Immunoprecipitation and immunofluorescence. However, the optimal concentrations should be determined individually. The antibody recognizes a ~110-140 kDa of HDAC-9. A 75 kDa and a 60 kDa bands can also be detected in some tissue lysates.|
|Restrictions||For Research Use only|
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|Buffer||100 μg (0.5 mg/mL) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||The antibody solution should be gently mixed before use.|
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
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|Product cited in:||
Kaluza, Kroll, Gesierich, Manavski, Boeckel, Doebele, Zelent, Rössig, Zeiher, Augustin, Urbich, Dimmeler: "Histone deacetylase 9 promotes angiogenesis by targeting the antiangiogenic microRNA-17-92 cluster in endothelial cells." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 33, Issue 3, pp. 533-43, 2013
Yan, Cao, Reilly, Young, Garcia, Mishra: "Histone deacetylase 9 deficiency protects against effector T cell-mediated systemic autoimmunity." in: The Journal of biological chemistry, Vol. 286, Issue 33, pp. 28833-43, 2011
Maltepe, Krampitz, Okazaki, Red-Horse, Mak, Simon, Fisher: "Hypoxia-inducible factor-dependent histone deacetylase activity determines stem cell fate in the placenta." in: Development (Cambridge, England), Vol. 132, Issue 15, pp. 3393-403, 2005
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