anti-Human Histone Deacetylase 9 antibody for Western Blotting

Recommended Histone Deacetylase 9 Antibody (supplied by: Log in to see )

Antigen
Histone Deacetylase 9 (HDAC9) Antibodies
  • HD7
  • HD7b
  • HD9
  • HDAC
  • HDAC7
  • HDAC7B
  • HDAC9B
  • HDAC9FL
  • HDRP
  • MITR
  • AV022454
  • D030072B18Rik
  • HD7B
  • Hdac7b
  • Mitr
  • mKIAA0744
  • hdac9
  • zgc:73392
  • TWIST
  • HDAC9
  • DKFZp459E171
  • HDA09
  • HISTONE DEACETYLASE
  • histone deacetylase 9
  • RGD1310748
  • RGD1563092
  • hdac9-A
  • histone deacetylase 9
  • histone deacetylase 9b
  • histone deacetylase 9 S homeolog
  • HDAC9
  • Hdac9
  • hdac9b
  • HDA9
  • hdac9.S
Epitope
C-Term
23
13
13
12
11
7
7
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Guinea Pig, Human, Mouse (Murine), Sheep (Ovine)
146
33
26
8
5
3
3
3
2
2
2
2
1
1
1
1
Host
Rabbit
118
28
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
5
4
4
4
Application
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Western Blotting (WB)
135
86
67
44
41
25
16
10
3
2
1
Options
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Catalog No. ABIN2775576
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.08196 ABIN223304 WB Rabbit IgG Log in to see Polyclonal 3
3.08196 ABIN2775579 WB Rabbit Middle Region Log in to see Polyclonal 1
3.08196 ABIN4880758 ELISA FACS WB Mouse IgG1 AA 343-569 Log in to see 2B7C4 0
3.08196 ABIN2704894 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0
3.08196 ABIN1533308 IF IHC ELISA WB Rabbit IgG AA 1017-1066 Log in to see Polyclonal 0
3.08196 ABIN3031237 IF IP IHC ELISA WB Rabbit Ig Fraction AA 2-32 Log in to see Polyclonal 0
3.08196 ABIN3185018 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.08196 ABIN2736885 WB Rabbit IgG Log in to see Polyclonal 0
3.08196 ABIN1868347 ICC IHC WB Rabbit IgG AA 23-343 Log in to see Polyclonal 0
3.08196 ABIN1843860 IF/ICC IHC WB Rabbit Log in to see Polyclonal 0
3.08196 ABIN2462632 IHC ELISA WB Rabbit Log in to see Polyclonal 0
3.08196 ABIN2989781 WB Rabbit IgG Log in to see Polyclonal 0
3.08196 ABIN5579678 ELISA WB Mouse Log in to see 0
3.08196 ABIN6262192 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
3.08196 ABIN6269422 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
3.08196 ABIN634236 WB Rabbit Middle Region Log in to see Polyclonal 0
3.08196 ABIN630205 IHC WB Rabbit C-Term Log in to see Polyclonal 0
3.08196 ABIN2668321 WB Rabbit C-Term Log in to see Polyclonal 0
3.08196 ABIN5956859 ELISA IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
3.08196 ABIN5963529 WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Histone Deacetylase 9 (HDAC9) Antibodies
Epitope C-Term
(23), (13), (13), (12), (11), (7), (7), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Guinea Pig, Human, Mouse (Murine), Sheep (Ovine)
(146), (33), (26), (8), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(118), (28)
Clonality
Polyclonal
Conjugate Un-conjugated
(5), (5), (5), (4), (4), (4)
Application Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Western Blotting (WB)
(135), (86), (67), (44), (41), (25), (16), (10), (3), (2), (1)
Pubmed 4 references available
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Product details

Target Details Application Details Handling References Images
Sequence QVGAVKVKEE PVDSDEDAQI QEMESGEQAA FMQQVIGKDL APGFVIKVII
Predicted Reactivity Guinea Pig: 100%, Human: 100%, Mouse: 100%, Sheep: 100%
Characteristics This is a rabbit polyclonal antibody against HDAC9. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human HDAC9

Target Details

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Antigen
Alternative Name HDAC9 (HDAC9 Antibody Abstract)
Background Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC9 has sequence homology to members of the histone deacetylase family. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. HDAC9 may play a role in hematopoiesis.Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
Alias Symbols: DKFZp779K1053, HD7, HDAC, HDAC7, HDAC7B, HDAC9B, HDAC9FL, HDRP, KIAA0744, MITR, HD9, HD7b
Protein Interaction Partner: NOP2, MKI67, EIF6, RCC1, ADAR, TOP1, TMPO, FIP1L1, CCAR2, TPX2, RBM14, ZC3H11A, HDAC9, NUP93, SMC3, H1FX, TPR, HINT1, CTBP1, BCL6, Zfp521, TRIM29, TP53, MAML1, SMARCA4, PARP1, CEBPB, H3F3A, RL2, CAMK1, YWHAE, HDAC1, SFN, MEF2B, ANKRA2, RFXANK, PKD1, SYK,
Protein Size: 590
Molecular Weight 65 kDa
Gene ID 9734
NCBI Accession NM_014707, NP_055522
Pathways Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development

Application Details

Product details Target Details Handling References Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 590 AA

Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Product cited in:

Spallotta, Tardivo, Nanni, Rosati, Straino, Mai, Vecellio, Valente, Capogrossi, Farsetti, Martone, Bozzoni, Pontecorvi, Gaetano, Colussi: "Detrimental effect of class-selective histone deacetylase inhibitors during tissue regeneration following hindlimb ischemia." in: The Journal of biological chemistry, Vol. 288, Issue 32, pp. 22915-29, 2013

Milde, Oehme, Korshunov, Kopp-Schneider, Remke, Northcott, Deubzer, Lodrini, Taylor, von Deimling, Pfister, Witt: "HDAC5 and HDAC9 in medulloblastoma: novel markers for risk stratification and role in tumor cell growth." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 12, pp. 3240-52, 2010

Hang, Yang, Han, Cheng, Shang, Ashley, Zhou, Chang: "Chromatin regulation by Brg1 underlies heart muscle development and disease." in: Nature, Vol. 466, Issue 7302, pp. 62-7, 2010

Background publications

Han, He, Wu, Liu, Chen: "Mechanism of recruitment of class II histone deacetylases by myocyte enhancer factor-2." in: Journal of molecular biology, Vol. 345, Issue 1, pp. 91-102, 2004

Images

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Supplier Images
Western Blotting (WB) image for anti-Histone Deacetylase 9 (HDAC9) (C-Term) antibody (ABIN2775576) WB Suggested Anti-HDAC9 Antibody Titration: 1.25ug/ml Positive Control: A172 cell ...
Chromatin Immunoprecipitation (ChIP) image for anti-Histone Deacetylase 9 (HDAC9) (C-Term) antibody (ABIN2775576) Chromatin Immunoprecipitation (ChIP) Using HDAC9 antibody - C-terminal region and HC...
Immunohistochemistry (IHC) image for anti-Histone Deacetylase 9 (HDAC9) (C-Term) antibody (ABIN2775576) Rabbit Anti-HDAC9 Antibody Paraffin Embedded Tissue: Human alveolar cell Cel...
Western Blotting (WB) image for anti-Histone Deacetylase 9 (HDAC9) (C-Term) antibody (ABIN2775576) Host: Rabbit Target Name: HDAC9 Sample Tissue: Human Fetal Brain Antibody Dilut...
Immunohistochemistry (IHC) image for anti-Histone Deacetylase 9 (HDAC9) (C-Term) antibody (ABIN2775576) Human kidney