anti-Rat (Rattus) Dopamine Receptor D5 antibody for Immunohistochemistry

Recommended Dopamine Receptor D5 Antibody (supplied by: Log in to see )

Antigen
Dopamine Receptor D5 (DRD5) Antibodies
  • DRD5
  • DBDR
  • DRD1B
  • DRD1L2
  • D1B
  • D5R
  • Drd-5
  • Drd1b
  • Gpcr1
  • dopamine receptor D5
  • DRD5
  • Drd5
Epitope
2nd Extracellular Loop, AA 199-211
24
10
4
4
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
77
43
42
1
1
Host
Rabbit
61
28
13
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
65
38
35
19
18
13
9
3
1
1
1
1
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Catalog No. ABIN1742089
$ 832.43
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.85804 ABIN1048520 IHC IHC (p) Rabbit Extracellular Domain Log in to see Polyclonal 0
1 ABIN258727 ICC IF IHC WB Mouse IgG1 kappa C-Term Log in to see SG4-D1b 1
1 ABIN1872361 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6261367 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6261366 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4305948 ICC IF IHC WB Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0
1 ABIN200806 IHC WB Mouse IgG1 C-Term Log in to see 0
1 ABIN3022433 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN409742 ICC IF IHC WB Biotin Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0
1 ABIN409744 ICC IF IHC WB DyLight 488 Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0
1 ABIN4305950 ICC IF IHC WB DyLight 405 Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0
1 ABIN4268399 ICC IF IHC WB DyLight 755 Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0
1 ABIN4268400 ICC IF IHC WB DyLight 680 Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0
1 ABIN4268402 ICC IF IHC WB Alexa Fluor 405 Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0
1 ABIN4268404 ICC IF IHC WB Alexa Fluor 647 Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0
1 ABIN4268394 ICC IF IHC FITC Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0
1 ABIN4268392 ICC IF IHC WB DyLight 550 Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0
1 ABIN4268395 ICC IF IHC WB DyLight 350 Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0
1 ABIN4268403 ICC IF IHC WB Alexa Fluor 488 Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0
1 ABIN4268405 ICC IF IHC WB Alexa Fluor 700 Mouse IgG1 kappa C-Term Log in to see SG4-D1b 0

General

Antigen Dopamine Receptor D5 (DRD5) Antibodies
Epitope 2nd Extracellular Loop, AA 199-211
(24), (10), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(77), (43), (42), (1), (1)
Host Rabbit
(61), (28), (13)
Clonality
Polyclonal
Conjugate Un-conjugated
(5), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(65), (38), (35), (19), (18), (13), (9), (3), (1), (1), (1), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Sequence EEGWELEGRT ENC
Cross-Reactivity (Details) Mouse - AA 12-13 residues identical. Unlikely to recognize human samples.
Characteristics Peptide Confirmation:
Confirmed by amino acid analysis.

Buffer after reconstitution:
Phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.025 % NaN3.

Antibody concentration after reconstitution:
0.8 mg/mL

Preadsorption Control:
1 µg peptide per 1 µg antibody.

GPCR
Purification Affinity purified on immobilized antigen.
Immunogen Peptide corresponding to amino acid residues 199-211 of rat D5 dopamine receptor. 2nd extracellular loop.

Target Details

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Antigen
Alternative Name D5 Dopamine Receptor (DRD5 Antibody Abstract)
Background Synonyms:
DRD5

The D5 Dopamine Receptor (D5 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson's disease and schizophrenia. All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1 The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activita. The D5 receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex, hippocampus and striatum. In the periphery the D5 receptor has been localized in the adrenal cortex, kidney and intestinal tract. The exact physiological function of the D5 receptor subtype remains poorly understood. Studies with D5 receptor knock out mice have shown no overt alterations in locomotor or cognitive functions. However, knock out mice do develop severe hypertension suggesting a role of D5 receptor in the modulation of neuronal pathways regulating blood pressure responsea., 3 Anti-D5 Dopamine Receptor (extracellular) antibody can be used in wstern blot analysis and immunohistochemical applications, and recognizes D5 dopamine receptor from rat and mouse samples.
Gene ID 25195
UniProt P25115
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

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Application Notes Recommended Dilution:
WB: approx. 1:200,
IHC: approx. 1:100
Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Lyophilized
Reconstitution 0.2 mL deionized water.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
Storage 4 °C/-20 °C
Storage Comment The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, small aliquots should be stored at -20 °C or below. The further dilutions should be made using a carrier protein such as BSA (1 %).

References

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Background publications

Hollon, Bek, Lachowicz, Ariano, Mezey, Ramachandran, Wersinger, Soares-da-Silva, Liu, Grinberg, Drago, Young, Westphal, Jose, Sibley: "Mice lacking D5 dopamine receptors have increased sympathetic tone and are hypertensive." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 24, pp. 10801-10, 2002

Images

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Supplier Images
Western Blotting (WB) image for anti-Dopamine Receptor D5 (DRD5) (2nd Extracellular Loop), (AA 199-211) antibody (ABIN1742089) Western blot analysis of mouse brain membranes: 1. Anti-D5 Dopamine Receptor (extrace...
Western Blotting (WB) image for anti-Dopamine Receptor D5 (DRD5) (2nd Extracellular Loop), (AA 199-211) antibody (ABIN1742089) Western blot analysis of rat striatum membranes: 1. Anti-D5 Dopamine Receptor (extrac...
Immunohistochemistry (IHC) image for anti-Dopamine Receptor D5 (DRD5) (2nd Extracellular Loop), (AA 199-211) antibody (ABIN1742089) Expression of D5 Dopamine Receptor in rat striatum Immunohistochemical staining of pe...