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|Antigen||Dopamine Receptor D5 (DRD5) Antibodies|
|Epitope||2nd Extracellular Loop, AA 199-211 Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
Immunohistochemistry (IHC), Western Blotting (WB)
|1 reference available|
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|Cross-Reactivity (Details)||Mouse - AA 12-13 residues identical. Unlikely to recognize human samples.|
Confirmed by amino acid analysis.
Buffer after reconstitution:
Phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.025 % NaN3.
Antibody concentration after reconstitution:
1 µg peptide per 1 µg antibody.
|Purification||Affinity purified on immobilized antigen.|
|Immunogen||Peptide corresponding to amino acid residues 199-211 of rat D5 dopamine receptor. 2nd extracellular loop.|
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|Alternative Name||D5 Dopamine Receptor (DRD5 Antibody Abstract)|
The D5 Dopamine Receptor (D5 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson's disease and schizophrenia. All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1 The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activita. The D5 receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex, hippocampus and striatum. In the periphery the D5 receptor has been localized in the adrenal cortex, kidney and intestinal tract. The exact physiological function of the D5 receptor subtype remains poorly understood. Studies with D5 receptor knock out mice have shown no overt alterations in locomotor or cognitive functions. However, knock out mice do develop severe hypertension suggesting a role of D5 receptor in the modulation of neuronal pathways regulating blood pressure responsea., 3 Anti-D5 Dopamine Receptor (extracellular) antibody can be used in wstern blot analysis and immunohistochemical applications, and recognizes D5 dopamine receptor from rat and mouse samples.
|Pathways||Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity|
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WB: approx. 1:200,
IHC: approx. 1:100
|Restrictions||For Research Use only|
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|Reconstitution||0.2 mL deionized water.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
|Storage||4 °C/-20 °C|
|Storage Comment||The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, small aliquots should be stored at -20 °C or below. The further dilutions should be made using a carrier protein such as BSA (1 %).|
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Hollon, Bek, Lachowicz, Ariano, Mezey, Ramachandran, Wersinger, Soares-da-Silva, Liu, Grinberg, Drago, Young, Westphal, Jose, Sibley: "Mice lacking D5 dopamine receptors have increased sympathetic tone and are hypertensive." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 24, pp. 10801-10, 2002
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